摘要
背景:肺的功能和结构改变致肺动脉高压是导致肺心病的先决条件,中药肺心合剂能够有效降低肺动脉高压,但其机制尚未完全阐明。目的:比较不同剂量中药肺心合剂与硝苯地平对肺动脉高压大鼠肺血管重建的逆转效应。设计:以实验动物为研究对象的随机对照实验研究。单位:一所医科大学附属医院护理系、一所医学院附属医院、一所省级医院中医科。方法:利用野百合碱(50mg/kg)复制大鼠肺心病模型。雄性Wister大鼠60只,随机分为6组:正常对照组、模型组、肺心合剂低、中、高剂量组及硝苯地平组,每组10只。分别于制模后14d灌胃相应药物,连续用药8d。处死动物后,利用特殊染色结合病理图象分析方法,测定肺小动脉病理及其形态计量学改变。主要观察指标:①光镜观察及肺小动脉图象分析。②各组肺小动脉管壁中膜厚度,管壁中膜厚度与血管外径比值、管壁面积与血管总面积比值、管腔面积与血管总面积比值。结果:肺心合剂及硝苯地平均能明显减轻模型大鼠的肺血管重构(P<0.01),以肺心合剂高剂量组效果最好。但治疗组各指标仍未完全恢复到正常对照组水平。结论:肺心合剂能部分逆转肺血管结构重建,有效降低肺动脉高压。
BACKGROUND:Pulmonary hypertension induced by alternation of pulmonary function and structure is the prerequisite of pulmonary-cardiac disorder.Chinese herb,fenxin mixture(FXM) reduces effectively pulmonary hypertension, but the mechanism on which has not been completely explained yet. OBJECTIVE:To compare the reversal effects between feixin mixture and nifedipine on vascular remodeling in rats with pulmonary hypertension. DESIGN:Randomized controlled experimental study in which experimental animals are taken as the objects. SETTING:Nursing department of affiliated hospital of a medical university;a medical college affiliated hospital and department of traditional Chinese medicine of a provincial hospital. METHODS:Monocrotaline(MCT) (50 mg/kg) was used to duplicate pulmonary-cardiac model in rats.Totally 60 Wister male rats were randomized into 6 groups,namely normal control group, model group,FXM groups of low,middle and high dosages successively and nifedipine group,10 rats in each group.Since the 14th day of modeling,the corresponding medications were administrated with gastric infusion,continuously for 8 days.After the rats sacrificed, the special staining collaborating with pathological image analysis was used to determine the measurement alternations on path-morphology of pulmonary small artery. MAIN OUTCOME MEASURES:①Microscopic observation and image analysis on pulmonary small artery.②medial thickness(MT),MT%,vessel wall square(VWS)/total vessel square(TVS),cavity square(CS)/TVS of pulmonary small artery in rats of every group. RESULTS:Both feixin mixture and nifedipine alleviate pulmonary vascular remodeling remarkably(P< 0.01),of which,the effect was the best in feixin mixture group of high dosage.But,the every index in experimental groups had not recovered to the level in normal control group completely. CONCLUSION:Feixin mixture reverses partially the remodeling of pulmonary vascular structure and reduces effectively pulmonary hypertension.
出处
《中国临床康复》
CSCD
北大核心
2005年第15期240-241,F003,共3页
Chinese Journal of Clinical Rehabilitation
