摘要
目的:构建远缘链球菌细胞分裂蛋白相关基因ftsK敲除菌株。方法:首先根据已知的远缘链球菌的ftsK基因序列,设计PCR引物,扩增并克隆了ftsK内部结构基因,亚克隆于自杀质粒后,通过同源重组替换野生型ftsK基因。结果:经过western杂交及southern杂交鉴定,证实重组质粒已插入细菌的基因组中。结论:成功构建远缘链球菌ftsK基因敲除菌株,为研究ftsK在致龋过程中细菌的扩增所起的作用奠定了基础。
AIM:To construct the mutant of Streptococcus sobrinus ftsK gene.METHODS:The structure gene of ftsK was amplified and cloned by PCR with the primers derived from the genome of S.sobrinus .Some of the bases were deleted by double digestion.Wide type gene was replaced through homologous recombination with a suicide vector containing the deleted gene.RESULTS:Western blot and southern blot confirmed that the suicide vector with aim fragment had inserted into the genome of wild typy S.sobrinus.CONCLUSION:The mutant of Streptococcus sobrinus ftsK gene is successfully constructed in the present study.
出处
《牙体牙髓牙周病学杂志》
CAS
2005年第5期251-254,共4页
Chinese Journal of Conservative Dentistry
基金
由第四军医大学创新工程资助(编号CX02A012)
关键词
fisK
同源重组
ftsK
homologous recombination
