摘要
目的:研究消化道疾病伴发复发性口腔溃疡(Recurrentaphthousstomatitis,RAU)患者,口腔内是否存在幽门螺杆菌(Helicobacterpylori,Hp)的感染,探讨Hp与RAU发病的关系。方法:运用聚合酶链反应技术(polymerasechainreaction,PCR)及尿素酶实验(rapidureasetest,RUT),检测246例患者唾液及胃黏膜中的Hp。并进一步运用单链构象多态性分析技术(Single-StrandConformationPolymorphism,SSCP),鉴定同一个体唾液和胃黏膜Hp菌株。结果:RUT实验检测246例患者胃黏膜Hp阳性率为32.93%(81/246)。3种引物PCR检测RAU患者唾液和胃黏膜Hp的阳性率均高于非RAU患者,且差异显著。对胃黏膜和唾液标本均呈阳性的29例患者PCR阳性产物分别经SSCP分析,其中24对胃黏膜和唾液标本的SSCP带型基本一致。结论:两种检测方法,以PCR特异性、灵敏性更高。同一个体的口腔和胃内存在同种Hp菌株的可能性较大,但并不排除混合性感染的可能。提示Hp和RAU的发病有一定的关联。
AIM:To investigate the relationship between Helicobacter pylori and recurrent aphthous stomatitis.METHODS:The distribution of Hp in the saliva and gastric mucosa of 246 patients with alimentary canal disease and recurrent aphthous stomatitis by polymerase chain reaction assay.The products were futher processed for SSCP.RESULTS:32.93%( 81/246) of RUT patients were caught up Hp in the gastric mucosa.The Hp- positive rate of the major RAU was enhanced obviously to non-RAU( P <0.05).PCR/SSCP analysis showed 82.76%(24/29) identity of the strains of Hp in the saliva and gastric mucosa.In five patients,the SSCP banding pattern in the Hp from saliva was inconsistent with that from gastric biopsy specimens.CONCLUSION:Among the two testing methods,the PCR is the more differential and sensitive one.It showed that oral cavity is an important parasitical place of Hp.Hp perhaps is one of the pathogen to RAU and it is indicated that the relationship between Hp and RAU is close clinically.But need further studies.
出处
《牙体牙髓牙周病学杂志》
CAS
2005年第5期274-278,共5页
Chinese Journal of Conservative Dentistry
基金
广东省自然科学基金资助(010620)
