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直肠癌中细胞外信号调节激酶和微血管计数的关系探讨 被引量：5

The Study on Relationship between ERK and Microvessel Count in Rectal Carcinoma
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摘要 目的:探讨细胞外信号调节激酶ERK-1、ERK-2在直肠癌中的蛋白表达水平和微血管计数的关系及其在直肠癌发生发展中的意义。方法:应用蛋白印迹法检测62例直肠癌及其邻近正常直肠粘膜中ERK-1和ERK-2蛋白的表达情况;Ⅷ因子相关抗原抗体免疫组化法测定微血管计数。结果:直肠癌中ERK-1、ERK-2的蛋白表达水平及微血管计数明显高于正常直肠粘膜;微血管计数较高的直肠癌其ERK-1、ERK-2的表达水平也明显增高。结论:ERK-1、ERK-2在直肠癌中呈现高表达,刺激血管内皮生长因子的增高,加速肿瘤微血管的生成,从而促进直肠癌的发生发展。 Objective: To study the relationship between ERK and microvessel count, Investigate its effect in the development of rectal carcinoma. Methods: Western blot was used to measure the expression of ERK-1 and ERK-2, immunohistochemistry was used to count the microvessel in 62 rectal carcinoma. Results: ERK-1?ERK-2 protein levels and microvessel count were increased in the rectal carcinoma compared with those in normal tissues. Conclusion: Overexpression of ERK may increase the level of vascular endothelial growth factor and microvessel count, playing an important role in the development of rectal carcinoma.
作者 陈维荣 王宇红 蔡高阳
机构地区 汕头大学医学院第二附属医院普外科
出处 《中国肿瘤临床》 CAS CSCD 北大核心 2004年第8期428-430,共3页 Chinese Journal of Clinical Oncology
基金 广东省卫生厅科研基金资助(编号:A2002449)
关键词 细胞外信号调节激酶 微血管 直肠癌 Extracellular signal-regulated kinases Microvessel Rectal carcinoma
分类号 R735.3 [医药卫生—肿瘤]
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参考文献10

  • 1[1]Licato LL, Brenner DA. Analysis of sigualing protein kiinase in human colon or colorectal carcinomas[J]. Dig Dis Sci, 1998, 43(7):1454～1464
  • 2[2]Kiyokawa E, Takai S, Tanaka M, et al. Overcxpression of ERK,an EPH family receptor protein tyrosine kinase, in various, in various human tumors[J]. Cancer Res, 1994, 54(14): 3645～3650
  • 3[3]Blenis J. Growth-regulated signal transduction by MAP kinase and reks[J]. Cancer Cells, 1991, 3(11): 445～449
  • 4[4]Rice P1, Goldberg RJ, Ray EC, et al. Inhibition of extracellular signalregulated kinase 1/2 phosphorylation and induction of apotosis by sulindac metabolites[J]. Cancer Res, 2001, 61 (4): 1541～1547
  • 5[5]Qiao D, Stratagouleas ED, Martinez JD. Activation and role of mitogen-activated protein kinases in deoxycholic acid-induced apoptosis[J]. Carcinogenesis, 2001, 22(1): 35～41
  • 6[6]Iwase T, Tanaka M, Suzuki M, et al. Identification of protein-tyrosine kinase genes preferentially exposed in embryo stomach and gastric cancer [J]. Biochem biophys Res Commun, 1993, 194(2):698～705
  • 7[7]Klemke RL, Cai S, Ciannini AL, et al. Regulation of cell motility by mitogen-activated protein kinase [J].J Cell Biol, 1997, 137(2):481～492
  • 8陈维荣,郑志超,吴祝东,李廷汉.微血管计数与直肠癌临床病理特点和预后关系的探讨[J].中国肿瘤临床,2001,28(5):383-384. 被引量：5
  • 9[9]Jung YD, Nakano K, Liu W, et al. Extracellular signal-regulated kinase activation is required for up-regulation of vascular endothelial growth factor by serum starvation in human colon carcinoma cells[J]. Cancer Res, 1999, 59(19): 4804～4807
  • 10[10]Jung YD, Kim MS, Shin BA, et al. EGCG, a major component of green tea,inhibits tumor growth by inhibiting VEGF induction in human colon carcinoma cells[J]. Br J Cancer, 2001, 84(6): 844～850

共引文献4

同被引文献48

  • 1霍艳英,李刚,王莹,张开泰,何欣蓉,刘敏丽,项晓琼,胡迎春,吴德昌.Smad7基因对胞外信号调节激酶磷酸化水平的调控[J].生物技术通讯,2005,16(3):239-241. 被引量：4
  • 2鲍同柱,郑启新,胡长耀.转化生长因子β超家族与肿瘤的研究进展[J].国外医学（物理医学与康复学分册）,2005,25(2):87-90. 被引量：4
  • 3王莉,霍艳英,张开泰,王莹,项晓琼,胡迎春,余刚,李刚,米粲,吴德昌.BEP2D细胞恶性转化过程中Smad7基因对MAPK信号通路的调控[J].癌症,2005,24(9):1080-1084. 被引量：10
  • 4赵杨,赵林,郭科军,温放.ERK蛋白在上皮性卵巢癌中的表达及意义[J].中国医科大学学报,2006,35(5):520-521. 被引量：1
  • 5Krueger JS, Keshamouni VG, Atanakova N, et al. Temporal and quantitative regulation of mitogen activated protein kinase (MAPK) modulates cell motility and invasion [ J ]. Oncogene,2001,20(31 ) :4 209
  • 6Bajo AM, Schally AV, Krupa M, et al. Bombesin antagonists inhibit growth of MDA-MB435 estrogen-independent breast cancers and decrease the expression of the ErbB-2/ HER-2 oncoprotein and c-jun and c-fos oncogenes [ J ]. Proc Natl Acad Sci USA,2002,99(6) :3 836
  • 7Ding L,Xu J, Luo X,et al. Gonadotropin releasing hormone and transforming growth factor beta activate mitogen-activated protein kinase/extraeellularly regulated kinase and differentially regulate fibroneetin, type 1 collagen, and plasminogen activator inhibitor-1 expression in leiomyoma and myometri- al smooth muscle cells[ J]. J Clin Endocrinol Metab,2004, 89(11) :5 549
  • 8Kamaraju AK,Roberts AB. Role of Rho/ROCK and p38 MAP kinase pathways in transforming growth factor-beta-mediated Smad-dependent growth inhibition of human breast carcinoma cells in vivo[J]. J Biol Chem,2005,280(2):1 024
  • 9Xie S,Sukkar MB,Issa R,et al. Regulation of TGF-beta 1- induced connective tissue growth factor expression in airway smooth muscle cells [ J ]. Am J Physiol Lung Cell Mol Physiol,2005,288( 1 ) :L68
  • 10Neubauer K, Knittel T, Aurisch S, Fellmer P, Ramadori G. Glial fibrillary acidic protein-a cell type specific marker for Ito cells in vivo and in vitro. Journal of Hepatology , 1996, 24(6): 719-730.

引证文献5

二级引证文献14

中国肿瘤临床
2004年 第8期

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