摘要
应用蔗糖密度梯度离心技术从蓝氏贾第鞭毛虫感染者的粪便中分离纯化贾第虫包囊，以此包囊免疫家兔，获得免疫血清，经大肠杆茵吸收后建立斑点免疫结合试验，检测粪中贾第虫特异性抗原。以此法检测３９例贾第虫感染者粪便，有３６例出现阳性斑点，阳性检出率为９２．３％。以超声粉碎的贾第虫包囊抗原为标准，３６例阳性标本中，有３５例标本贾第虫特异性抗原蛋白含量分布在３９～６９ｎｇ／ｍＬ范围内，１例为１３８ｎｇ／ｍＬ。
A dot immunobinding assay for detection of Giardia lamblia specific anti- gen(GSA)in
fecal eluates was developed. The rabbit antiserum to Giardia lamblia cystwhich had been
absorbed with Escherichia coli was used in the test,The positive rate in microscopically
confirmed cases was 92.3%(36/39).The estimated amount of antigen found in almost positive
samples ranged from 39 ng to 69 ng Giardia protein per millil-itre ,except one with 138 ng per
millilitre compared with results of serially diluted cystantigen.The amount of soluble cyst
antigen as low as 3.9ng/mL could still be detected by this test.
出处
《中国兽医学报》
CAS
CSCD
1994年第1期77-80,共4页
Chinese Journal of Veterinary Science
关键词
斑点免疫
鞭毛虫
抗原
粪便
dot immunobinding
assay
Giardia lamblia
antigen
feces
