摘要
比较了应用改良Ｔｙｒｏｄｅ′ｓ液（简称Ｔ，对照组）与０．６％ＢＳＡ－ＴＣＭ１９９（简称ＢＴ）作为受精液（实验１）、这两种受精液的不同配比作为受精液（Ｔ：ＢＴ分别为３：１、１：１和１：３；Ｔ为对照组，实验２）和在不同受精时间采用不同受精液（先用Ｔ，ＩＶＦ２４ｈ后改用ＢＴ或ＴＣＭ１９９＋１０％发情牛血清，实验３），对经体外成熟培养（ＩＶＭ）后的牛卵母细胞体外受精（ＩＶＦ）效果的影响。结果，应用成分复杂的ＢＴ作为受精液，可以提高受精卵体外囊胚发育率（５５．２％，对照组为３７．１％，Ｐ＜０．０１）；但受精卵卵裂率（４１．８％）却极显著地低于对照组（７９．４％，Ｐ＜０．００１）；当应用Ｔ：ＢＴ为３：１和１：１的混合液作为受精液时，受精卵仍保持较高的囊胚发育率（分别为５３．１％和５７．４％，对照组为４２．２％）；而受精卵卵裂率（分别为７９．１％和６７．０％）虽比对照组（８４．５％）低，但明显高于单纯用ＢＴ组（４１．８％），从而使总的囊胚率得到提高。而在ＩＶＦ２４ｈ后用ＢＴ或ＴＣＭ１９９＋１０％发情牛血清将原先受精用的Ｔ液更换，再继续进行２０ｈ或４６ｈ的受精培养，则对体外受精无任何改善作用。结果说明，在现行使用的、成分简?
The
experiments were separately conducted to determine the effects offertilization media which
included modified Tyrode's solution(T),BSA+TCM-199(BTCM)and T +BTCM mixtures in different
proportions(3:1,1:1 and 1:3,V/V)on the fertilization of bovine oocytes in vitro. The results
showed that the embryos at 2-8-cell stage fertilized in BTCM media had significantly high
capacity to develop to theblastocyte stage(55.8%)than those fertilized in modified Tyrode's
solution(37.1%),though the cleavage rate(CR) in BTCM group (41.8%)was much lower than that
in Tgroup(79.4%,P<0.001). When the oocytes were fertilized in T+BTCM mixtures in 3:1 or 1:1
proportion,not only were the development rate of blastocysts from 2-8-cellembryos kept at a
high level(53.7%and 57.4%respectively),but the oocytes'CR wasalso high(79.0%and
67.0%respectively). It showed that the quality of 2-8-cell em- bryos can be improved when the
bovine oocytes were fertlized in the medium containing0.6%BSA and 25%to 50%TCM-199, and
more blastocysts can be obtained in vitrc。
出处
《中国兽医学报》
CAS
CSCD
1994年第2期140-145,共6页
Chinese Journal of Veterinary Science
关键词
牛卵母细胞
体外受精
受精液
bovine oocyte
in vitro fertilization
fertilization nedium
embryo qual-ity
