摘要
经Ｌ１６（４５）正交试验选择，并经实验验证，鸡脾脏Ｔ淋巴细胞白细胞介素２（ＩＬ－２）体外诱生和活性检测的最优水平组合及适宜条件为２．５μｇ／ｍＬ伴刀豆蛋白Ａ（ＣｏｎＡ）、ＩＬ－２体外诱生时间２０ｈ、１×１０７／ｍＬ淋巴细胞、１０％小牛血清、靶细胞培养时间４８ｈ、４×１０６／ｍＬ靶细胞、靶细胞接触时间３６ｈ、５ｍｇ／ｍＬＭＴＴ、ＭＴＴ加入时间３ｈ和甲溶解时间２ｈ；胸腺Ｔ淋巴细胞ＩＬ－２体外诱生和活性检测的最优水平组合及适宜条件为５μｇ／ｍＬＣｏｎＡ、ＩＬ－２体外诱生时间４８ｈ，余同脾脏Ｔ淋巴细胞ＩＬ－２体外诱生及活性检测。比较试验表明，ＭＴＴ比色分析法和３Ｈ－胸腺嘧啶核苷（３Ｈ－ＴｄＲ）掺入法有显著的直线回归关系。
The IL-2
induction from chicken T lymphocytes and its activity test werestudied.It was shown that when
the spleenic T lymphocytes were used, the best consti-tution and optimum eonditions were as
follows :2.5 μg/mL Con A ,inductive time invitro 20 hours,4 ×106/mL target cells,interactive
time of target cells with IL-2 36hours,5mg/mL MTT,additive time of MTT 3 hours,dissolving time
of formazan particles 2 hours,In case of thymus T lymphocytes,based upon the selection of
orthogonaltest and verification by experiment, the constitution and conditions were identical
withthose in spleenic T lymphocytes but the Con A concentration was 5 mg/mL and induc-tive
time 48 hours. The comparative test indicated that there was significant linear re-gression
relation between the MTT colorimetric assay and the 3H-TdR incorporation.
出处
《中国兽医学报》
CAS
CSCD
1994年第2期159-163,共5页
Chinese Journal of Veterinary Science
关键词
鸡
T淋巴细胞
IL-2
MTT比色分析
chicken
T lymphocyte
IL-2
MTT colorimetric assay
3H-TdR in-Corporation
