摘要
以２６６ｎｍ及３８２ｎｍ波长处喹乙醇的色谱峰高比为一定值，且不随浓度变化而改变作为喹乙醇的定性依据，测定了死亡鸡肝和肾组织中喹乙醇的含量。用乙腈—乙酸乙酯（３／２，Ｖ／Ｖ）提取组织中的喹乙醇。高效液相色谱条件：ＡｌｔｅｘＵｌｔｒａｓｐｈｅｒｅｔｍＯＤＳ－Ｃ１８分离柱（２５０ｍｍ×４．６ｍｍ）；流动相为甲醇—水（１５／８５，Ｖ／Ｖ）；流速１．０ｍＬ／ｍｉｎ；紫外检测波长２６６ｎｍ或３８２ｎｍ。组织中的药物在０．４～１２．８μｇ／ｇ的浓度范围内的标准曲线呈线性。５只死亡鸡肝组织中喹乙醇的浓度分别是７．８６、１０．４２、１４．７９、２４．１０及２４．９１μｇ／ｇ；而肾组织中的浓度分别是１１．２０、１５．４３、２２．５８、２８．１９及３０．２５μｇ／ｇ，证实为喹乙醇中毒。
The simple and sensitive reversed-phase
high performace liqtud chro-matographic procedure was developed for the demonstration of
olaquindox toxication inchickens,and the quantitative determination of olaquindox in chicken
tissues (liver andkidney).A reversed-phase ODS-C18(5 μm)column fitted with a UV detector
was usedfor analysis of olaquindox, The wavelength of detector used for the determination of
o-laquindox was 266 or 382 nm。 Olaquindox in chicken tissues was extracted with
acetoni-trile-ethyl acetate(3 ∶ 2,V/V) ,and the extract was evaporated to dryness。 The
mobilephases consisted Of methanol-water(15∶ 85,V),and flowed at a rate of 1.0mL/min。The
calibration curve was linear in the range from 0.4μg/g to 12.8 μg/g tissue (r=0. 998 1-0.999 2).
The drug recovery of the extracted procedure of olaquindox was 75. 21±5. 17%(X±S). The
tissue concentrations of olaquindox in five died chickenswere 7. 86,10. 42,14. 79,24. 10 and
24.91 μg/g in liver, respectively, and 11.20,15. 43,22.58,28.19 and 30.25 μg/g in kidney,
respectively. The results indicated that the five chickens died of olaquindox toxication。 The
main reasons of Olaquindox toxication in poultry were discussed in this paper。
出处
《中国兽医学报》
CAS
CSCD
1994年第4期394-397,共4页
Chinese Journal of Veterinary Science
