摘要
采用水蒸汽蒸馏－乙醚同时萃取装置对抗白背飞虱品种ＲａｔｈｕＨｅｅｎａｔｉ（ＲＨＴ），ＩＲ６４，Ｎａｂｅｓｈｉ和感虫对照品种ＴＮ１稻株进行挥发性次生物质提取．浓缩所得精油，经“气谱－质谱”（ＧＣ－ＭＳ）联机检测出６４个有机的化学组分，其中３６种主要分子结构为：９种醇．９种醛．７种酮４种酯，３种烷烃．２种酸，烯烃和杂环化合物各１种。２－已烯醛、β－紫罗兰酮、叶醇（反式）、叶绿醇、３，５．５－三甲基－３－环已烯－１－酮和水杨酸甲酯的含量较高。将蒸馏提取物回喷在ＴＮ１稻株上，白背飞虱对抗虫和感虫稻株蒸馏提取物的趋性没有显著差异。嗅觉仪测定飞虱的趋性结果表明。单体化合物苯甲醇和已烯醛比对照丙酮更易吸引白背飞虱。在回喷ＴＮ１蒸馏提取物、丙酮溶液和未处理的ＴＮ１稻株上，白背飞虱分泌的蜜露量明显地较在Ｎａｂｅｓｈｉ和ＩＲ６４蒸馏提取物处理过的ＴＮ１稻株上分泌的高。白背飞虱从掺有蒸馏提取物和挥发物单体的营养液中吸食的量均比从单纯营养液中的少；抗虫品种ＲＨＴ蒸馏提取物对白背飞虱拒食作用最强，β－紫罗兰酮和已烯醛次之。
The rice plants of susceptible variety TN1 and resistant varietics Rathu Heenati (RHT).IR64and Nabeshi to the whitebacked planthopper, Sogatella furcifera,were extracted using a device of steam distillation-simultaneous extraction with diethyl ether. The oily extracts were obtained through further extraction and condensation of steam distillatcs and analyzed by gas chromatography-mass spectrometry (GC-MS). The analytical results indicate that the corn position of 36 secondary volatiles among 64 detected compounds was 9 alcohols. 9 aldehydes. 7 ketones, 4 esters, 3 hydrocarbons. 2 acids. 1 olefin and heterogeneous ring compound. The volatile chemicals with larger quantities were 2-hexenal. irisone. trans-hexenol.phytol. 3.5.5-trimethyl-3-cyclohexadien-1-ol and methyl salicylate. The two-choice tests or S. furcifera on TN1 plants treated with steam distillates extracts (SDE) indicate that the preference of S. furcifera to SDE of both resistant and susceptible varieties did not differ significantly. The tests of S. furciferaorientation by an olfactometer reveals that phenacyl alcohol and hexenal attracted significantly more in insects than check acetone. S. furcifera excreted significantly more honeydew on the untreated TN1 and TN1 plants treated with acetone and SDE of TN1 than on TN1 plants treated with SDEs of Nabeshi and IR64.S.furcifera ingested less from the nutritional solution incorporated with SDEs and the selected volatiles than from nutritional solution alone. The SDE of resistant RHT possessed the strongest antifceding effect to S.furcifera.following by β-irisone and hexenal.
出处
《中国水稻科学》
CAS
CSCD
北大核心
1994年第4期223-230,共8页
Chinese Journal of Rice Science
基金
浙江省自然科学基金
关键词
白背飞虱
抗虫性
水稻
品种
feeding response
insect resistance: orientation
secondary volatiles
Sogatella furcifera
