摘要
The enzymatic(Alcalase 3.0T) hydrolysis process of isolated soybean protein(ISP) and radius (distribution) of its hydrolysis product were investigated and characterized by laser light scattering(LLS) and ζ-potential methods. With the increase of hydrolysis time, the average hydrodynamic radius of hydrolyzed ISP (aggregates) decreased from 160 nm to 110 nm, and the hydrodynamic radius distribution of ISP (aggregates) broadened, and the ζ-potential decreased from -24 mV to about -37 mV. The enzymatic(Alcalase 3.0T)hydrolysis reaction finished within 3h. FTIR spectra of ISP before and after the (hydrolysis) indicate that the hydrolyzates of ISP had much more non-(associated) O—H and N—H groups than native ISP.
The enzymatic(Alcalase 3.0T) hydrolysis process of isolated soybean protein(ISP) and radius (distribution) of its hydrolysis product were investigated and characterized by laser light scattering(LLS) and ζ-potential methods. With the increase of hydrolysis time, the average hydrodynamic radius of hydrolyzed ISP (aggregates) decreased from 160 nm to 110 nm, and the hydrodynamic radius distribution of ISP (aggregates) broadened, and the ζ-potential decreased from -24 mV to about -37 mV. The enzymatic(Alcalase 3.0T)hydrolysis reaction finished within 3h. FTIR spectra of ISP before and after the (hydrolysis) indicate that the hydrolyzates of ISP had much more non-(associated) O—H and N—H groups than native ISP.
出处
《应用化学》
CAS
CSCD
北大核心
2005年第5期557-559,共3页
Chinese Journal of Applied Chemistry
基金
国家自然科学基金(20374025
20476040)资助项目
关键词
大豆分离蛋白
水解作用
光散射
isolated soybean protein,hydrolyzation,laser light scattering
