摘要
目的:探讨慢性坐骨神经挤压损伤大鼠脊髓组织中胶质纤维酸性蛋白和磷酸化细胞外信号调节激酶表达的改变。方法:实验于2003-06/09在军事医学科学院毒物药物研究所一室完成。雄性SD大鼠12只,随机分为2组,假手术组和慢性坐骨神经挤压损伤组,每组6只。按Bennett法,大鼠腹腔注射戊巴比妥钠40mg/kg后,假手术组暴露右侧坐骨神经不结扎,慢性坐骨神经挤压损伤组在该神经主干部位,用4-0铬制羊肠线松结扎4道。两组术后7和14d以von-Freyfilaments和冰水测定触痛及冷刺激反应,采用免疫组化法测定慢性坐骨神经挤压损伤大鼠脊髓组织中胶质纤维酸性蛋白和磷酸化细胞外信号调节激酶表达的改变。结果:12只大鼠全部进入结果分析。①术后7和14d,慢性坐骨神经挤压损伤组(术侧)触痛阈值分别下降80.3%和84.8%,冷刺激反应分别升高309.4%和336.2%,组间比较差异有显著性(P<0.01)。②术后14d,慢性坐骨神经挤压损伤大鼠双侧脊髓背角胶质细胞胶质纤维酸性蛋白和磷酸化细胞外信号调节激酶表达均有增加,手术侧分别增加246.4%和173.6%,与假手术组比较差异有显著性(P<0.01)。结论:慢性坐骨神经损伤后,中枢神经系统胶质细胞外信号调节激酶/丝裂原活化蛋白激酶通路激活,可能参与慢性神经痛的发病过程。
AIM:To evaluate the expression changes of glial fibrillary acidic protein(GFAP) and phosphorylated extracellular signal regulated kinase(pERK) in spinal cord of rats with chronic constriction injury(CCI) of sciatic nerve.METHODS:The experiment was conducted in the First Department of Institute of Toxicology and Pharmacy,Chinese Academy of Military Medical Sciences from June to September 2003.Twelve male SD rats were randomly divided into 2 groups:sham operation group(n=6) and CCI group(n=6).After phenobarbital sodium 40 mg/kg was injected into abdominal cavity of all the rats by Bennett method,the rats in CCI group were placed with four loose 4- 0 chromic catgut ligatures around the nerve shaft of the right sciatic nerve;however,the right sciatic nerve of the rats in the sham operation group was exposed but not deligated.Hyperalgesia and cold stimulation test were performed by using von Frey filaments and ice cold water at 7 and 14 days after operation.The expression of GFAP and pERK in spinal cord of CCI rats was detected by immunohistochemiical technique.RESULTS:All the 12 animals were included in the result analysis.① At 7 and 14 days after operation,the threshold of haphalgesia was decreased by 80.3% and 84.8% in the operated sides of CCI group,and cold stimulation test increased by 309.4% and 336.2% respectively.There was significant different in the inter group comparison(P< 0.01).② The expressions of GFAP and pERK of axonal fibers in the bilateral spinal dorsal horn of CCI rats were both increased at 14 days after operation,by 246.4% and 173.6% in the operated sides respectively,significantly different from those in the sham operation(PCONCLUSION:CCI of sciatic nerve may activate ERK/MAPK(mitogen activated protein kinase) pathways of astrocytes in the central nervous system,and ERK/MAPK pathways may play an important role in the genesis of chronic neuropathic pain.
出处
《中国临床康复》
CSCD
北大核心
2005年第17期110-112,共3页
Chinese Journal of Clinical Rehabilitation
基金
国家自然科学基金资助课题(20302015)~~
