摘要
背景:细胞内过氧化物质增加产生的氧化损伤可以加速癫痫发作的频率,而通过氧化还原作用消耗过氧化物,可起到对中枢神经元的保护作用。目的:分析癫痫患者血浆及红细胞中抗氧化剂水平的变化。设计:非随机同期化的平行对照(相互对照、空白对照)。单位:一所大学医院的检验科、精神科、药剂科。对象:2000-03/12中南大学湘雅二医院神经内科门诊确诊的癫痫患者(癫痫疾病组)32例,均符合国际抗癫痫联盟的诊断及分类标准,且未经过任何治疗。男17例,女15例,年龄27～59岁。同期选择本院神经内科住院癫痫患者(治疗组)26例,符合国际抗癫痫联盟的诊断及分类标准,接受了1年多的苯巴比妥类药物的治疗,在此期间无癫痫急性发作的症状;男16例,女10例,年龄24～58岁。正常对照组为随机选取本院门诊体检人员39例,体检各项提标均在正常参考范围内;男23例,女16例。上述均为知情同意者。方法:3组对象于清晨8:00～9:00空腹采集静脉血2mL。采用酶偶联连续监测法测定红细胞的内谷胱甘肽还原酶,谷胱甘肽过氧化物酶,过氧化氢酶活性的测定。采用邻苯三酚自氧化比色法测定红细胞中超氧化物歧化酶活性。采用改良TBA比色法测定红细胞中丙二醛的含量。同时测定血红蛋白浓度和红细胞孵育渗透脆性(以溶血百分率计算)。
BACKGROUND:Oxidative injury due to increased intracellular peroxides leads to more frequent onset of epileptic seizure,and the consumption of peroxides through oxidation reduction played an important role in protection of the central nervous system neurons.OBJECTIVE:To analyze the changes of antioxidants in plasma and erythrocyte of patients with epilepsy.DESIGN:Nonrandomized,paralleled,concurrent controlled study.SETTING:Department of laboratory,psychology,and pharmacy in a university hospital.PARTICIPANTS:Totally 32 epileptic patients were diagnosed in the Department of Neurology,Xiangya Second Hospital,Central South University between March and December 2000 according to the diagnostic and classification criteria of International League Against Epilepsy.The patients consisted of 17 male and 15 female subjects aged from 27 to 59 years,all of whom denied previous anti epileptic drug exposure(epileptic group). Another 26 concurrent epileptic patients,including 16 male and 10 female subjects aged from 24 to 58 years,confirmed according to the same criteria and treated at the Department of Neurology were also recruited in this study,who reported a history of anti epileptic therapy with phenobarbiturals for more than one year without acute onset of epileptic seizures during this period(treatment group). Totally 39 normal controls including 23 male and 16 female subjects were enrolled from those receiving routine health examinations with normal physical indexes.Informed consent was obtained from all these participants.METHODS:Fasting venous blood(2 mL) were collected from each subject at 8:00- 9:00 am.Enzyme coupled continuous monitoring was employed to determine the activity of erythrocyte glutathione reductase(EGR),glutathione peroxidase(GSH Px),and catalase(CAT).Pyrogallol autoxidation colorimetry was used to determine the activity of erythrocyte superoxide dismutase(SOD). The level of erythrocyte malonaldehyde(MDA) was examined with improved TBA colorimetry.Meanwhile hemoglobin(HB) concentration and osmotic fragility of incubated erythrocytes(expressed as the hemolytic percentage) were examined.Plasma vitamin A,C,and E were also determined with high performance liquid chromatography.Plasma ceruloplasmin(CER) was examined with immunodiffusion assay.MAIN OUTCOME MEASURES:The activity of EGR,GSH Px,SOD,CAT,content of MDA,erythrocyte hemolytic percentage and the levels of CER and vitamin A,C,E.RESULTS:Only one patient failed to complete the treatment.Erythrocyte MDA content,hemolytic percentage,activity of GSH Px,and CAT,and CER in epileptic group[(176.5± 12.0) μ mol/L,(3.32± 0.95)% ,(1 503.6 ± 130.0) nkat/g,(75.3± 14.6) K/g,(487.0± 25.4) mg/L] and treatment group[(129.5± 7.4) μ mol/L,(1.52± 0.20)% ,(1 323.6 ± 95.0) nkat/g,(64.2± 10.1) K/g,(345.0± 15.2) mg/L] were significantly higher than those in the control group(t=2.46- 3.89,P< 0.05],but the activity of erythrocyte GR and SOD and the content of plasma vitamin A,E,C[(101.7± 13.3) nkat/g,(20.2± 0.8) μ kat/g,(1.18± 0.83) μ mol/L,(20.7± 4.5) μ mol/L,(20.6± 3.6) μ mol/L,and(213.4± 45.0) nkat/g,(28.5± 0.9) μ kat/g,(3.14± 0.30) μ mol/L,(40.5± 6.6) μ mol/L,(38.1± 5.1) μ mol/L] were significantly lower than those in the control group(t=2.46- 2.97,P< 0.05).The activity of GR and SOD and plasma vitamin A,C content of normal control group were significantly higher than those in the epileptic group [(161.7± 25.0) nkat/g,(26.7± 0.9) μ kat/g,(2.09 ± 0.35) μ mol/L,(26.2± 4.1) μ mol/L,t=2.46- 2.66,PCONCLUSION:The decrement of intracellular GSH Px and SOD,and plasma levels of vitamin A,C,E,and CER in epileptic patients can be indicative of the activity of the free radicals during the onset of epileptic seizures.
出处
《中国临床康复》
CSCD
北大核心
2005年第17期245-247,共3页
Chinese Journal of Clinical Rehabilitation
