摘要
目的研究胃癌中骨桥蛋白(OPN)mRNA的表达及其与临床病理特征之间的关系,阐明骨桥蛋白在胃癌侵袭和转移过程中的作用。方法应用半定量RT PCR方法,检测OPNmRNA在胃癌组织中的表达和相对含量,分析OPNmRNA的相对含量与胃癌临床病理特征的关系。结果66例胃癌组织中,50例标本可扩增出330bp的条带,OPNmRNA的阳性表达率为75.8%(50/66)。20例正常胃黏膜组织OPNmRNA的表达全部为阴性。OPNmRNA的表达与肿瘤侵袭深度、肿瘤直径、淋巴结转移程度及有无远处转移有关,与肿瘤的分化程度无关。66例患者随访10～27个月(平均16个月)。OPNmRNA表达阳性组50例中复发15例,表达阴性组16例中仅复发1例(P=0.05);OPNmRNA表达阳性组中死亡10例,表达阴性组无一例死亡(P=0.05)。结论胃癌中,骨桥蛋白mRNA的表达增高。OPNmRNA的高表达反映了胃癌病情的进展,并且与患者的预后有关。骨桥蛋白在胃癌的远处转移过程中可能起着重要的作用。
Objective To investigate the expression of osteopontin mRNA and its correlation with clinicopathologic features of gastric cancer and elucidate its role in tumor invasion and distant metastasis. Methods The expression of OPN mRNA was detected by semi-quantitive RT-PCR. The relationship between the relative content of OPN mRNA and clinicopathologic features of gastric cancer was analyzed. Results In 66 cancer tissue samples, a 330 bp band was detected in 50 cases, the positive rate of OPN mRNA expression was 75.8% (50/66). The expression in all 20 cases of normal gastric mucosa was negative. The expression was associated with the depth of tumor invasion, diameter, lymph node metastasis and but had no correlation with differentiation grades. The 66 patients were followed up for 10～27 months (mean 16 months). The OPN mRNA expression positive group (50 cases) had recurrence in 15 patients and the negative group (16 cases) had only 1 case with recurrence (P=0.05); 10 patients died in OPN mRNA expression positive group but no patient died in OPN staining negative group (P=0.05). Conclusion OPN mRNA is over-expressed in gastric cancer. It reflects the progression of disease and association with poor prognosis of gastric cancer. OPN may play an important role in the process of distant metastasis in gastric cancer.
出处
《中华肿瘤杂志》
CAS
CSCD
北大核心
2005年第5期292-295,共4页
Chinese Journal of Oncology
关键词
骨桥蛋白mRNA
胃癌
表达
肿瘤转移
预后
Osteopontin mRNA
Stomach neoplasms/pathology
Neoplasm metastasis
Prognosis
