摘要
目的寻求一种有效的体外分离、纯化成人睾丸间质细胞的方法。方法利用4个梯度(60%、34%、26%、21%)的Percoll液分离、纯化成人睾丸间质细胞。对分离的细胞进行细胞学观察、锥虫蓝染色、3β羟基类固醇脱氢酶(3βHSD)染色、分泌睾酮能力和绒毛膜促性腺激素(hCG)对分离睾丸间质细胞的作用等检测。结果通过该方法能够获得高纯度(>90%)的成人睾丸间质细胞,并通过上述实验检测证实分离、纯化的睾丸间质细胞有良好的分泌睾酮能力。结论采用该方法对成人睾丸间质细胞进行初步的分离和纯化是简单有效的。
Objective: To obtain a simple and effective method to isolate and purify adult Leydig cells. Methods: The testes of human adults were digested and then the density gradient centrifugation of the cells was performed with four different Percoll concentrations (60%, 34%, 26%, 21%) to isolate Leydig cells, whose characteristics were identified by cytological observation staining, 3β-HSD staining and detection of hCG and testosterone secretion. Results: High-concentration (>90%) purified Leydig cells were acquired, and many identification experiments demonstrated the adequate testosterone secretory function of the isolated and purified Leydig cells. Conclusion: This method is easy and efficient for the isolation and purification of adult Leydig cells.
出处
《中华男科学杂志》
CAS
CSCD
2005年第5期356-358,共3页
National Journal of Andrology
基金
广东省科技计划项目基金(2003C34008)
广东省医学科学研究基金(B2004034)
广州市科学技术委员会基金(2000J002.02)资助
