摘要
用平板红细胞凝集（ＨＡ）试验对鸡产蛋下降综合症（ＥＤＳ－７６）病毒进行了快速定性检测，并与微量ＨＡ试验进行了对比。结果表明，当病毒ＨＡ滴度在５×２１２及其以上时，在１０～２５℃范围内于半分钟可与１０％鸡红细胞（ＲＤＣ）发生极明显的大片凝集。当病毒ＨＡ滴度在５×２１０以下时，则凝集片明显较小，且反应时间在２分钟以上。这种凝集现象可被ＥＤＨ－７６阳性血清特异性抑制。此法可用于对大批量样品中高效价病毒，特别是种毒的初步选筛。
Abstract Egg Drop Syndrome 1976 (EDS-76) virus was rapidly detected by plate hemagglutination (HA) test for quantitative analysis and by micro-HA test for quantitative determination. Different shapes occured in plate HA test were corelated with the virus titers obtained from micro-HA test.When virus were able to completely agglutinate an equal volume of 10% chicken erythrocyle suspension and the agglutinated shapes were obvious in plate test at 10～25℃ within 30 seconds, their geometric mean titers (GMT) of micro-HA test were more than 5×212.If the agglutinations of the samples were not apparent or needed more than 2 minutes to occur in plate test, their micro-HA titers were less thans×210. The agglutination of the samples could be specially inhibited by EDS-76 antisera. This method is considered suitable for a rapid initial screening system to get higher titer viruses especially for selection of seed viruses from a large number of original samples.
