摘要
将较大批量的眼镜蛇粗毒依次通过ＳｅｐｈａｄｅｘＧ７５，ＤＥＡＥ－ｃｅｌｌｕｌｏｓｅＤＥ５２和Ｂｉｏ－ＧｅｌＰ２００柱层析可高效地分离纯化眼镜蛇蛇毒因子（ＣＶＦ），５０ｇ粗毒可制备电泳纯ＣＶＦ９６０ｍｇ，产率大大高于既往文献报道，整个制备过程仅需时数日。通过ＳＤＳ聚内烯酰胺凝胶电泳测得其分子量为２２６０００，其溶血活性从抗补体活性均与文献报道相近。所以，本实为一种经济，高效的实验室大量制备ＣＶＦ的方法。
An efficient method for fractionationand purification of cobra venom factor (CVF) fromthe venom of Naja naja atra by successive chromato-graphy on Sephadex G75, DEAE-cellulose DE52 andBioGel P200 columns was reported in detail.Homogeneity of the CVF obtained was shown as asingle band by both immuno-electrophoresis andpolyacrylamide disc-electrophoresis. The yield was1.92%, i.e. 960 mg obtained from 50 g of crudevenom, much higher than that ever reported. Thewhole procedure of fractionation was completed with-in 3 days. Its relative molecular weight was estimatedto be 226000 composed of 3 subunits by SDS-polyacrylamide gel electrophoresis. lts hemolytic andanticomplementary activities were found as potent asreported else where. The newly developed method is ex-pected to be economically significant for mass produc-tion of CVF in laboratory for chronic in vivopharmacologic experiments.
出处
《中国药理学与毒理学杂志》
CAS
CSCD
北大核心
1994年第4期271-275,共5页
Chinese Journal of Pharmacology and Toxicology
