摘要
目的为研究供体脑死亡对供体外周器官的影响,建立一种无脑电图仪监测下渐进式大鼠脑死亡模型。探讨大鼠脑死亡模型制作要点、成功率以及血流动力学参数。方法57只Fisher大鼠行动脉插管、气管插管、诱导建立脑死亡模型,取肾前维持机械呼吸6h。将平均动脉压(MAP)>80mmHg的脑死亡大鼠,取其肾脏原位移植给Lewis大鼠。另外10只Fisher大鼠作为正常对照组供体。结果57只Fisher大鼠中45只(78.9%)在6h的机械性人工呼吸观察中,MAP始终在正常水平,可作为脑死亡供体。10只正常对照组大鼠,MAP虽呈下降趋势,但仍在正常水平。来自脑死亡大鼠的肾脏移植后再灌注时间(22±7)min与来自对照组的(5±3)min相比差异具有显著性(P<0.001)。其余12只大鼠被剔除出研究。结论无脑电图仪监测下,渐进式建立大鼠脑死亡模型,可达到与标准方法建立的脑死亡模型相似的保持正常血压的效果,可用于改善脑死亡供体器官质量和移植物功能。
Objective To study the effect of brain death on peripheral organs, we established a gradual onset brain death model in rats without electroencephalograph monitoring. Methods Fifty-seven Fisher rats were intubated, tracheotomized and induced to establish brain death models. Rats were mechanically ventilated for 6 hours prior to kidney retrieval for transplantation. The harvested kidneys from suitable brain death rats (mean arterial blood pressure, MAP>80 mmHg) were orthotopically engrafted to Lewis rats. Additional 10 rats were used as controls.Results Forty-five of the 57 rats(78.9%) could maintain MAP(80～100 mmHg) during 6-hour mechanical ventilation and be used as brain death donors. Twelve rats were excluded from the study. The MAP in 10 control rats demonstrated a declining tendency. However, it still remained at the normal level. Significant difference was present in the reperfusion time[(22±7) min in brain death donors versus(5±3) min in controls, P<0.001)]. Conclusion The established gradual onset brain dead model in rats without electroencephalograph monitoring can reach the similar effect of maintaining normal blood pressure as described in a standard model. This model seems to be appropriate for transplantation-associated studies in improving of organ quality of brain dead donor and graft function.
出处
《广东医学》
CAS
CSCD
北大核心
2005年第6期768-770,共3页
Guangdong Medical Journal
基金
广东省医学科研基金资助项目(编号:A2005348)
德国科学研究协会(DeutscheForschungsgemeinschaft
DFG)基金资助项目(编号:PR578/2-1)
