摘要
目的 研究大鼠肺纤维化中单核细胞趋化蛋白- 1 (MCP -1) 和转化生长因子- β(TGF- β) 的表达及己酮可可碱对它们的影响。方法 采用气管内灌注博来霉素的方法建立大鼠肺纤维化模型, 设对照组、模型组和己酮可可碱治疗组, 每组12只, 再各分为第7天和第28天组。测定28 d时肺组织中羟脯氨酸含量和溶胶原活性以判断纤维化程度及治疗效果, 同时用免疫组化法观察7、28 d时MCP 1和TGF -β在肺组织中的表达变化。结果 模型组羟脯氨酸含量显著增高、溶胶原活性下降, 而己酮可可碱治疗组与对照组比较无明显差异。与对照组比较, 模型组MCP- 1 和TGF- β的表达显著增高(均P<0. 01), 己酮可可碱治疗组也有所增高, 但均明显低于模型组(均P<0. 01)。结论 己酮可可碱可抑制鼠肺纤维化, 调节MCP- 1和TGF- β的表达可能是己酮可可碱抑制鼠肺纤维化的机制之一。
Objective To study the expression of monocyte chemoattractrant protein-1 (MCP-1) and transforming growth factor-β (TGF-β) in pulmonary fibrosis of rats and the effects of pentoxifylline on them.Methods The model of rat pulmonary fibrosis was established by intratracheal injection of bleomycin. Rats were randomly divided into 3 groups (n=12 in each group): the pulmonary fibrosis group,the pentoxifylline treatment group and the control group. Each group was divided into the 7 thday subgroup and the 28 th day subgroup. The hydroxyprolline and the collagenolytic activity of pulmonary homogenates were measured for evaluation of the degree of pulmonary fibrosis. At the same time,the expression of MCP-1 and TGF-β in lung tissue was detected by immunohistochemistry.Results As compared with control group,the hydroxyprolline was obviously increased and the collagenolytic activity was decreased in fibrosis group,but there was no significant difference between the treatment group and the control group. The expression of MCP-1 and TGF-β in fibrosis group were significantly increased as compared with control group (both P<0.01). The expression levels of MCP-1 and TGF-β were also increased in the treatment group,but significantly lower than in fibrosis group (both P<0.01).Conclusion One of the mechanisms by which pentoxifylline can prevent pulmonary fibrosis of rats might be that pentoxifylline regulates the expression of MCP-1 and TGF-β.
出处
《华中科技大学学报（医学版）》
CAS
CSCD
北大核心
2005年第2期192-195,共4页
Acta Medicinae Universitatis Scientiae et Technologiae Huazhong
基金
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