摘要
采用ＨＰＬＣ测定血清中环丙氟哌酸的浓度。流动相为１４％甲醇，８％乙腈，７８％磷酸盐缓冲液（其中含０．３％氢氧化四丁基铵，０．０２ｍｏｌ／Ｌ磷酯二氢钾，ｐＨ＝３．０±０．０２）。采用反相色谱柱，２７７ｎｍ紫外检测。二氯甲烷提取，以二氟沙星为内标物，血样中药物检测限是１０ｎｇ／ｍｌ，回收率９８．８％，日内相对标准偏差是３．５％，日间相对标准偏差是１．３８％。通过兼用ＨＰＬＣ和生物检定法测定健康受试者的血清药物浓度，对两种分析方法进行比较，将用两种方法测定的结果（ｎ＝８０）进行线性回归，直线的斜率是０．９１，相关系数是０．９５９（Ｐ＜０．０１）。
A simple accurate and reproducible high performance liquid chromatographic proce-dure was developed for the determination of ciprofloxacin(CIP)in serum。 The mobile phase con-tained 14%methanol , 8%acetonitrile and 78%phosphate buffer solution(0.3%tetrabutylam-moinium hydroxide,0. 02mol/L potassium dihydrogenphosphate, pH=3.0±0. 02 phase analyti-cal column, with ultraviolet detection).This method involved a simple one-step extraction proce-dure of dichloromethane for CIP in serum, and utilized difloxacin as internal standard. The de-tection limit for blood level wsa 10ng/ml. The mean recovery of CIP in the method was 98.8%。The ralative standard deviation was 3.5%for within day precision and 1.38%for day to day pre-cision。 High performance liquid chromatography and the bioassay procedure were comparedthrough assay of a series of actual volunteers′s specimens (n=80).The pregression of the bioassayand high pertformance liquid chromatographic assay data gave a linear relationship with a slope of0.91 and a correlation coefficient of 0.959(P<0.01)。
出处
《中国药学杂志》
CSCD
北大核心
1994年第8期483-485,共3页
Chinese Pharmaceutical Journal
关键词
环丙氟哌酸
高效液相色谱
生物检测
ciprofloxacin
high performance liquid chromatography
bioassay
