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小麦籽粒硬度及其分子遗传基础研究回顾与展望 被引量：37

Review and Prospect of Wheat Kernel Hardness and Its Molecular Genetics Basis
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摘要 籽粒硬度是最重要的小麦品质性状之一,是市场分级和定价的重要依据。随着硬度测试方法的日趋完善,其分子遗传基础的研究逐步加快。硬度主要受位于5D染色体短臂上一个主效基因和多个微效基因控制,Pina和Pinb是形成小麦籽粒硬度的基础。PINA蛋白的缺失或编码PINB蛋白的基因突变均造成小麦胚乳质地变硬。中国目前该项研究较少,与国外相比仍有很大差距,本文着重阐述了小麦胚乳结构及硬度的生化和遗传基础,旨在为我国小麦籽粒硬度的研究提供理论依据。 Kernel hardness, controlled by a major gene located on the short arm of chromosome 5D, is one of the most important wheat quality characteristics and determines marketing and classification of common wheat. With the development of hardness measuring, the molecular genetic basis of kernel hardness has rapidly progressed. It is known that Pina and Pinb form the molecular basis of wheat kernel hardness and lacking of PINA or mutation in Pinb caused wheat endosperm become hard. Endosperm structure and genetic basis of hardness are summarized in order to provide useful information in understanding the formation of grain hardness.
作者 陈锋 李根英 耿洪伟 夏兰芹 夏先春 何中虎
机构地区 中国农业科学院作物科学研究所/国家小麦改良中心
出处 《中国农业科学》 CAS CSCD 北大核心 2005年第6期1088-1094,共7页 Scientia Agricultura Sinica
基金 国家863计划(2002AA207003) 国家自然基金(30260061) 973重点发展研究规划(2002CB11130)
关键词 籽粒硬度 基础研究 展望 回顾 小麦品质性状 分子遗传基础 小麦胚乳 测试方法 基因控制 主效基因 基因突变 染色体 A蛋白 PIN Common wheat Kernel hardness Friabilin Puroindoline Pina Pinb
分类号 S512.1 [农业科学—作物学] G311 [文化科学]
  • 相关文献

参考文献42

  • 1Rahman S, Jolly C J, Skerritt J H, Wallosheck A. Cloning of a wheat 15 kDa grain softness protein (GSP). GSP is a mixture of puroindoline-like polypeptides. Europe Journal of Biochemistry, 1994, 223: 917-925.
  • 2Greenwell P, Schofield J D. A starch granule protein associated with endosperm softness in wheat. Cereal Chemistry, 1986, 63: 379-380.
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  • 9Perretant M R, Cadalen T, Charmet G, Sourdille P, Nicolas P, Boeuf C, Tixier M H, Branlard G, Bernard S. QTL analysis of bread-making quality in wheat using a doubled haploid population. Theoretical and Applied Genetics, 2000, 100: 1 168-1 175.
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二级参考文献21

  • 1Greenwell P, Schdleld J D. A starch granule protein associated with endosperm softness in wheat. Cereal Chemistry, 1986, 63: 379-380.
  • 2Gautier M F, Alay R, Lullien V, Joudrier P. Nucleotide sequence of a Cdna encoding the wheat ( Triticum durum Desf. ) CM2 protein.Plant Molecular Biology, 1991,16:333 -334.
  • 3Iadlien V, Alary R. Guirao A, Joudrier, Gautier M F. Isolation and nucleotide sequence of a cDNA clone encoding the bread wheat( Triticum aestivum L. ) CM17 protein. Plant Molecular Biology,1991, 17:1 081 - 1 082.
  • 4Blochet J E, Chevalier C, Forest E, Pebay-Peyroula E, Gautier M F, Joudrier P, Pzolet M M, Marion D. Compolete amino acid sequence of puroindoline, a new and cycteine-rich protein with a unique tryptoph-rich domain isolated from wheat endosperm by Triton X-114 phase partioning. FEBS Letter, 1993, 329: 336-340.
  • 5Gautier M F, Aleman M E, Guirao A, Marion D, Joudrier P.Triticum aestivnm puroindolins, two basic cystine rich seed proteins..cDNA analysis and developmental genes expressin. Plant Moleadar Biology, 1994, 25: 43-57.
  • 6Jolly C J, Rahman S, Kortt A A, Higgins T J V. Characterization of the wheat Mr 15000grain-softness pritein' and analysis of the relationship between its accumulation in the whole seed and grain softhess. Theo~ical and Applied Gendics, 1993, 86: 589-597.
  • 7Rahman S, Jolly C J, Skenitt J H, Wallosheck A. Cloning of a wheat 15-kDa gran softness protein (GSP) . GSP is a mixture of puroindoline-like polypeptides. Europe Journal of Biochemistry,1994, 223: 917-925.
  • 8Jolly C J. Glenn G M, Rahman S. Gsp-I genes are linked to the gnfin hardness locus (Ha) on wheat chromosome 5D. Proceedings of the National Academy of Science USA, 1996, 93:2 408- 2 413.
  • 9Mon'ison W R, Greenwell P, Law C N, Sulaiman B D. Occurrence of friabilin, a low molecular weight protein associated with grain softness, on starch granules isolated from some wheats and related species. Journal of Cereal Science, 1992, 15: 143-149.
  • 10Giroux M J, Morris C F. A glycine to serine change in puroindoline b is asssociated with wheat grain hardness and low levels of starch-surface friabilin. Theoretical and Applied Gentics, 1997, 95: 857-864.

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引证文献37

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中国农业科学
2005年 第6期

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