摘要
用免疫组织化学法，在大鼠下丘脑、丘脑、大脑皮层、海马、第三脑室室管膜及脉络膜等脑组织细胞内发现有胰岛素存在，一些细胞的核内也似有胰岛素存在。免疫电镜结果再次证实了上述观察。用放射免疫法发现大鼠各脑区提取液中胰岛素含量高低不等。摄食活动可引起脑内胰岛素含量增加，尤以下丘脑为最，中枢胰岛素的作用之一即可能为参与机体能量平衡的调控。同时测定血浆、脑脊液及脑组织的胰岛素含量，结果表明脑内胰岛素不可能由血浆经脑脊液而进入，尚有其它来源。
Using the immunohistochemical method,insulin was proved to exist in cells of hypothalamus,thalamus,cerebral cortex,hippocampus,ependyma of third ventricle and choroid of rat,including some of the neuronal nuclei,and was further confirmed by immunoelectromicroscopy.The insulin concentrations in rat brain differed in various regions. Simultaneous determinations of insulin concentrations in brain.CSF and blood showed that the brain insulin could not originate from blood through CSF.The participation of brain insulin in regulating of body energy balance at hypothalamus was illustrated and discussed.
出处
《中国医学科学院学报》
CAS
CSCD
北大核心
1994年第6期434-437,共4页
Acta Academiae Medicinae Sinicae
基金
国家自然科学基金
关键词
胰岛素
中枢胰岛素
免疫组织化学
免疫电镜
insulin
brain insulin
immunohistochemical method
immunoelectromicroscopy
