摘要
目的研究蚯蚓提取物地龙2号对实验性大鼠肝纤维化的α肌动蛋白(αSMA)、转化生长因子β1(TGFβ1)、尿激酶型纤溶酶原激活物(uPA)及Ⅰ型纤溶酶原激活抑制物(PAI1)蛋白表达的影响。方法52只雄性Wistar大鼠,随机分成6组:正常组(N);阴性对照组(NC),皮下注射花生油,灌单蒸水;模型组(M),皮下注射CCl4,每日单蒸水灌胃;阳性对照组(PC),造模同时用秋水仙碱0.1mg·kg-1·d-1灌胃;地龙大剂量组(Eh),造模同时用地龙2号50mg·kg-1·d-1灌胃;地龙小剂量组(El),造模同时用地龙2号25mg·kg-1·d-1灌胃。8周末处死大鼠,用免疫组化法检测各组大鼠肝脏组织中αSMA、TGFβ1、uPA及PAI1的表达情况。结果地龙2号大、小剂量组与模型组相比,αSMA、TGFβ1、uPA及PAI1的表达均显著降低,且uPA、PAI1与αSMA及TGFβ1表达下降呈明显的正相关。结论地龙2号可能通过抑制肝纤维化大鼠肝组织中肝星状细胞的活化,降低TGFβ1、uPA及PAI1的表达,发挥抗肝纤维化作用。
Objective To investigate the effects of earthworm extractions (E2) on the expressions of α-SMA,uPA,TGFβ_1 and PAI-1 of hepatic fibrosis in rats. Methods Fifty-two male Wistar rats were randomly divided into six groups:normal group (N), normal control group (NC), model group (M), positive control group (PC), high dose of E2 group (Eh) and low dose of E2 group (El). The rats in group of M, PC, Eh and El were subcutaneously injected with CCl_4 for 8 weeks to induce liver fibrosis, and the rats in group NC were injected with peanut oil only.Group PC rats were administrated with colchicine. Rats in group Eh and El were administrated with different dose of E2. At the end of 8 weeks,all survived rats were sacrificed humanely, liver specimens were preserved in 10% buffered paraformaldehyde and dehydrated in a graded alcohol series. Following xylene treatment, the specimens were embedded in paraffin blocks and cut continuously into 4μm thick sections, and the expressions of α-SMA,TGFβ_1,uPA and PAI-1 were observed by immunohistochemistry technique.Results Compared to those in model group, the expressions of α-SMA,TGFβ_1,uPA and PAI-1 in animals of groups Eh and El were significantly lower and the decrease of uPA and PAI-1 was obviously positively correlated with that of α-SMA and TGFβ_1 in groups Eh and El. Conclusion E2 can inhibit obviously the formation of liver fibrosis induced by CCl_4 in rats, which may be relateed to inhibiting the activation of hepatic stellate cells and decreasing the proteinic expressions of TGFβ_1,uPA and PAI-1.
出处
《江苏医药》
CAS
CSCD
北大核心
2005年第6期443-445,F003,共4页
Jiangsu Medical Journal
基金
江苏省中医药管理局科研项目(Z019)
