摘要
目的:观察125I粒子近距离照射对肝癌细胞及肝癌移植瘤生长的影响。方法:用四唑盐(MTT)法检测125I粒子近距离照射对BEL-7402细胞增殖的影响;流式细胞术检测125I粒子近距离照射对BEL-7402细胞周期和凋亡的影响;观察125I粒子近距离照射后肝癌移植瘤的生长曲线,并计算抑瘤率。结果:125I粒子近距离照射48h后细胞增殖抑制率为(16·72±3·23)%,96h为(36·60±7·14)%。照射48h后细胞位于G0~G1、S、G2~M期的比率分别为(40·47±0·64)%、(38·18±0·91)%和(21·35±0·65)%,而对照组分别为(54·47±1·17)%、(35·83±0·41)%和(9·71±1·27)%;照射48h后细胞凋亡指数为(7·31±1·41)%,对照组为(0·69±0·14)%。125I粒子植入裸鼠肿瘤近距离照射28d后抑瘤率为66·72%。结论:125I粒子近距离照射可抑制BEL-7402肿瘤细胞增殖,阻滞细胞于G2~M和S期,促进凋亡,显著延缓肝癌移植瘤生长,可用于原发性肝癌的治疗。
OBJECTIVE:To evaluate the effect of ^(125)I seed brachytherapy to BEL-7402 cell line and xenografts. METHODS: Proliferation of BEL-7402 cells was measured after ^(125)I seed brachytherapy by using MTT method; the effect of ^(125)I seed brachytherapy on cell cycle distribution and apoptosis was investigated by FCM; the antitumor efficacy of ^(125)I seed brachytherapy in mouse xenograft liver tumors was observed. RESULTS: The proliferation inhibition rates after ^(125)I seed brachytherapy for 48 h and 96 h were (16.72±3.23)% and (36.60±7.14)%, respectively. The distribution of cell cycle in G_0-G_1,S,G_2-M phase after ^(125)I seed brachytherapy for 48 h were (40.47±0.64 )%, (38.18±0.91)% and (21.35±0.65)%, while in the controller were (54.47±1.17)%, (35.83±0.41)% and (9.71±1.27)%, respectively. The apoptosis index after ^(125)I seed brachytherapy for 48 h was (7.31±1.41)%, while in the controller was (0.69±0.14)%。The volume inhibition rate of xenograft tumor in nude mice was 66.72% after ^(125)I seeds were implanted into tumors for 28 days. CONCLUSIONS: ^(125)I seed brachytherapy can inhibit proliferation of BEL-7402 cell line, change cell cycle distribution, cause G_2-M and S phase arrest, accelerate apoptosis and delay the growth of xenograft tumor in nude mice. ^(125)I seed brachytherapy may be used in the treatment of primary liver cancer.
出处
《肿瘤防治杂志》
2005年第10期750-752,共3页
China Journal of Cancer Prevention and Treatment
