期刊文献+

腺嘌呤致大鼠雄性不育发病机理的实验研究 被引量:16

EXPERIMENTAL STUDY ON THE MECHANISM OF ADENINE-MODELED INFERTILITY IN MALE RATS
原文传递
导出
摘要 目的探讨转化生长因子β(TGFβ)超家族的细胞内信号转导分子Smad2、Smad4蛋白在腺嘌呤所致雄性不育的大鼠睾丸内定位、分布及作用机制。方法应用免疫组织化学SABC法检测正常成年及腺嘌呤(300mg kg)灌胃后第7、14、21d大鼠睾丸中Smad2、Smad4的定位和分布,并通过图像分析系统进行统计学分析,同时应用放射免疫法测定血清中睾酮(T)、促黄体激素(LH)和促卵泡激素(FSH)的含量。结果Smad2免疫阳性产物位于实验大鼠各级生精细胞及支持细胞的胞质内,Samd4则位于间质细胞的胞质内;灌胃后第21d大鼠睾丸中Smad2、Smad4表达明显高于正常成年及7、14d大鼠,同时,血清中T较正常成年及7、14d大鼠明显降低,LH、FSH明显增高。结论腺嘌呤所致雄性不育可能与Smad2在大鼠睾丸生精细胞中表达增强及Smad4在睾丸间质细胞中表达增强,直接或间接影响睾丸的生精功能有关。 Objective To study the localization, distribution of Smad 2 and Smad 4 proteins in rat testis which are intracellular signaling molecules of adenine-modeled infertility. Methods The localization, distribution of Smad 2 and Smad 4 proteins were detected by immunohistochemical SABC method and the expression levels of Smad 2 and Smad 4 proteins were detected by quantitative measurement through image analyzing system. The contents of(testosteone,T), (lutelinizint hormone,LH), and (follicle-stimulating hormone,FSH) were detected by radioimmunoassay. Results After 7 and 14 days of adenine-modeled infertility, the expression levels of Smad 2 and Smad 4 were increased, but no significant difference. After 21 days of adenine-modeled infertility, the expression levels of Smad 2 and Smad 4 were increased significantly. The contents of T were decreased significantly, but the contents of LH and FSH were increased significantly.Conclusion The strong expression of Smad 2 protein in spermatogenic cell and Smad 4 protein in Leydig cell can directly and indirectly influence the spermatogenetic function of testis in adult rat. Adenine-modeled infertility is related to these elements.;
出处 《解剖学报》 CAS CSCD 北大核心 2005年第3期278-283,共6页 Acta Anatomica Sinica
基金 国家自然科学基金资助项目(39870109 30370750) 全军医药卫生科研基金资助项目(98M106 01MA183)
关键词 腺嘌呤 不育 SMAD2 SMAD4 免疫组织化学 放射免疫 大鼠 Adenine Infertility Smad 2 Smad 4 Immunohistochemistry Radioimmunoassay Rat
  • 相关文献

参考文献6

二级参考文献59

  • 1费仁仁,戴文平,戴巾,陈晖,朱斌,邓锦松.大鼠精核蛋白的纯化[J].生物化学杂志,1994,10(5):557-560. 被引量:11
  • 2晏子友,皮持衡,徐友妹,陈人骏.慢性肾衰及其中医证型与尿及血渗量的关系[J].江西中医药,1995,26(2):22-23. 被引量:9
  • 3郑平东,朱燕俐.用腺嘌呤制作慢性肾功能衰竭动物模型[J].中华肾脏病杂志,1989,5(6):342-344. 被引量:115
  • 4费仁仁,陈晖,戴文平,刘平,陈啸梅,薛社普.雷公藤单体T4对大鼠变态期精子细胞核蛋白转换的影响[J].生殖与避孕,1996,16(1):46-48. 被引量:11
  • 5BarryM Brenner.Brenner & Rector’s The Kindey[M].北京:科学出版社,2001.1820--1823.
  • 6陈允硬 周华文 张友康.实用检验医学指南[M].上海:上海科学普及出版社,2000.160.
  • 7冯仁丰.实用医学检验学[M].上海:上海科学技术出版社,2000.313.
  • 8Berry M Brenner.Brenner & Rector’s The Kindey[M].北京:科学出版社,2001.1563--1585,1820--1868,1236.
  • 9[1]Rodriguez JB & Barcia CM.Spontaneous germ cell death in the testis of the adult rat takes the form of apoptosis: re-evaluation of cell types that exhibit the ability of die during spermatogenesis.Cell Prolif,1996,29(1): 13~ 31.
  • 10[2]Yin Y, Dewolfk WC & Morgentaler AJW.Experimental cryptorchidism induces testicular germ cell apoptosis by p53-dependent and independent pathways in mice.Biol Reprod, 1998,58(3):492~7.

共引文献78

同被引文献110

引证文献16

二级引证文献82

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部