摘要
目的检测精子的RNA,以保证后续实验有可靠、准确的结果。方法收集成年男性活力正常的精子,提取其RNA,应用微流路芯片高压凝胶电泳检测精子总RNA,以健康成人淋巴细胞总RNA作对照。结果检测发现在人类精子中有大量的基因表达。精子RNA的电泳图出现两个峰,两峰的位置都较正常体细胞提前,先出的峰其形相对尖锐,后出的峰其形较宽,跨越5S,但仍呈典型的倒U形分布,两峰的比值,远远大于2∶1,在其他位置无明显吸收峰。结论应用微流路芯片高压凝胶电泳,可进行精子RNA简单、直观地检测和质量控制。
Objective Detecting of spermatozoal total RNAs by laboratory on chip gel electrophoresis so that it could provide better total RNAs for the sequent experiments, and spur the development of spermatozoal molecular biology. Methods Sperms of healthy adults were collected and then total RNAs were extracted by RNeasy mini kit(QIAGEN), detection and quality control were performed by loboratory on chip gel electrophoresis system. Meantime, the control RNAs were extracted from lymphocytes. Results It was found that there were a plenty of genes expressed in healthy sperms. Electrophoretic graphs showed that the total RNAs of spermatozoal had 2 bands which went ahead a little comparing to the normal somatic cells. The former peak appeared keenness, and the latter was broad and showed like a reversed U. The ratio of them was largely more than 2, no extra peaks were found in electrophoretic graph. Conclusion A simple,intuitionistic method to detect and control the quality of the healthy adults' spermatozoal total RNAs had been successfully constructed by using laboratory on chip gel electrophorosis.
出处
《解剖学报》
CAS
CSCD
北大核心
2005年第3期326-329,共4页
Acta Anatomica Sinica
基金
广东省自然科学基金资助项目(04020392)
2003年广东省医学科学技术研究基金(A2003443)
关键词
微流路芯片高压凝胶电泳
芯片上的实验室
精子RNA
Mocrofludic gel electrophoresis
Laboratory on chip microarray
Spermatozoal total RNAs
