摘要
用聚丙烯酰胺凝胶垂直平板电泳法分析了５—６０周龄的大鼠左心室肌凝蛋白重链同功酶（ｍｙｏｓｉｎｈｅａｖｙｃｈａｉｎｉｓｏｅｎｚｙｍｅｓ，ＭＩ）。结果表明：大鼠幼年期ＭＩ以高ＡＴＰ酶活性的Ｖ１形式存在（５周龄：Ｖ１＝９８．９２±１．４７％），随着年龄增长，Ｖ２（１０周龄：３．５４±２．４２％）和Ｖ３（１５周龄６．３１±３．４８％）依次出现并逐步增加，到２８周时Ｖ１、Ｖ２、Ｖ３分别为６１．３６±１１．７１％、２０．１１±４．９８％和１３．５２±５．３２％，随后基本稳定于该水平；以Ｖ１／Ｖ３为指标分析各周龄组大鼠，可见Ｖ１／Ｖ３具有增龄性的下降趋势，但均以Ｖ１占优势（６０周：Ｖ１／Ｖ３＝３．８５±０．９５）；同功酶谱变化以及它与左室重量相关分析表明，同功酶谱增龄性变化主要发生在大鼠生命的早期，并且这种变化可能与左室重量的增长有关。
Left ventricular
myocardium myosin heavy chain isoenzyme(MI) patterns of rats at 5 60week ages were
analysed by using pyrophosphate polyacrylamide gel electrophoretic technique. Tbe result
showed that there was an age-dependent shift in MI distribution toward the adult pat- tern. Left
ventricular MI was exclusively V1(98.92±1.47%)at 5 weeks of age, V2 was found at 10weeks(3.
54 ±2.42%) and V3 appeared at 15 weeks(6.31±3.48%)of age. Vl,V2 and V3 were61.36±
11.71%, 20.11±4.98% and 13.53±5.32% at 28 weeks.V1/V3 ratio decreased graduallywith the
growth of rat, but V1 remained predominant until 60 weeks(V1/V3∶3.85±0.95). Corre-lation and
regression analysis showed that there was linear correlation between V3 and leftventricular
weight.
出处
《中国应用生理学杂志》
CAS
CSCD
1994年第1期1-4,共4页
Chinese Journal of Applied Physiology
基金
国家自然科学基金
关键词
肌凝蛋白重链
同功酶
心肌
年龄
myosin heavy chain isoenzymes(MI)
age-dependent
left ventricular
myocardium
rat
