摘要
采用新生大鼠海马细胞进行分离培养，观察缺氧对海马培养细胞的影响以及降钙素基因相关肽（ＣＧＲＰ）的保护作用。结果表明：培养１２ｄ的细胞置于缺氧环境（９５％Ｎ２＋５％ＣＯ２）中４─２４ｈ后，随着缺氧时间延长，神经细胞由肿胀、胞膜受损乃至死亡，台盼蓝染色细胞数增多，乳酸脱氢酶（ＬＤＨ）和Ｋ＋漏出增加。经ＣＧＲＰ孵育的细胞缺氧后形态变化轻微，细胞死亡率以及ＬＤＨ和Ｋ＋漏出量均明显低于对照组。本结果表明，体外培养的海马神经元对缺氧甚为敏感。缺氧造成海马细胞严重损伤，ＣＧＲＰ对此有明显的保护作用。
The effects of anoxia on cultured hippocampal
cells and the protectivc effect of calcitonin gene-related peptide(CGRP)were studied.On day 12
after plating of the hippocampal cells fromnewborn rat,the cultures were exposed to anoxic
atmosphere(90 % N2+10% CO2)for 4-24hours.The results showed that the hippocampal neurons
became swollen,vacuolated and disinte-grated and virtually all neurons died after 24 h of
anoxic exposure. Following progressive anoxia,the release of lactate dehydrogenase (LDH)and
K+into the bathing medium obviously in-creased. In contrast with the control,the cultures
pretreated with CGRP showed better preserva-tion of both neuronal cell bodies and neurites and
higher numbers of surviving neurons.The val-ues of LDH and K+efflux in bathing medium
containing CGRP were significantly lower thanthose of controls.These results demonstrate that
the dissociated cultured hippocampal neuronsfrom newborn rat are extremely sensitive to and
seriously damaged by anoxia.Such damagecould be attenuated by CGRP,suggesting that CGRP
may protect neurons from damage duringanoxia.
出处
《中国应用生理学杂志》
CAS
CSCD
1994年第4期317-321,共5页
Chinese Journal of Applied Physiology
基金
国家自然科学基金资助项目中国科学院上海生理所低氧生理开放实验室资助项目
关键词
降钙素基因
相关肽
缺氧
海马
细胞培养
calcitonin gene-related
peptide
anoxia
hippocampus
cell culture
