摘要
授粉后２５－３０天的花生幼胚，去子叶后，在ＭＳ２（ＭＳ＋ＢＡ１．０ｍｇ／Ｌ＋ＣＨ３００ｍｇ／Ｌ＋ＰＶＰＰ０．３％＋椰计５０ｍｌ／Ｌ＋蔗糖４％＋琼脂０．８％）培养基中，置３００ｌｕｘ弱光和２０℃±１℃条件下，培养２１－２８天，能有效地产生丛生芽；在ＭＳ２中诱导培养后的去子叶幼胚的生长点切去，在改良无激素培养基（ＭＳ１）中培养１４天，转入ＭＳ３（ＭＳ＋ＢＡ６．０ｍｇ／Ｌ＋ＮＡＡ０．４ｍｌ／Ｌ＋Ｄ－生物素０．１ｍｇ／Ｌ＋ＣＨ３００ｍｇ／Ｌ＋酵母浸出物２００ｍｇ／Ｌ＋椰计５０ｍ／Ｌ＋ＰＶＰＰ０．３％＋蔗糖２．５％＋琼脂０．８％）培养基，置２５℃±１℃，３５００ｌｕｘ光照条件下培养２１－２８天，８０％的外植体分化出丛生芽，继而长成无根带叶小苗．．平均每外植体产生８．１个丛生芽，最多可达４０个．诱根后小苗移植田间８０％植株成活并开花结实。品种（系）间差异不显著。
An experiment using five peanut cultivars was conducted for plant regeneration via organogenesis from decotyledon-immature embryo cultuer. The decotyledon young embryoes were cultured on the induction medium (Modifed MS with BA 1. 0mg/IL+CH300mg/L+PV PP 0. 3%+coconut milk 50mL /L+sucrose4%+agarose0. 8 %, PH =5. 8)under dark of dim light at 20℃±1℃for21-28 days. The embryo germinating point was cut out or killed and the explants were then transfered to a secondary medium (with BA 0. 6mg/L+NAA 0. 4mg/L+biotin 0.1mg/L+CH 300mg/L+yeast extract 200mg/L+coconut milk 50mL/L+PV PP 0. 3%+sucrose 2. 5%+agarose 0. 8% .PH=5. 8)and cultured for 28 days.The rate of polyshoot-organogenesis explants was about 31% .and the average number ofshoots per explant was 6. 7. Roots were well induced on the root-inducing medium. The regenerated plants were tranfered to field plot and seeds from these plants were obtained. No significant difference in regeneration ratio was observed among the five cultivars used.
出处
《中国油料》
CSCD
北大核心
1994年第4期28-31,共4页
基金
湖北省自然科学基金
关键词
花生
去子叶幼胚
丛生芽
植株再生
Peanut
Decotyledon-immature embryo
Poly shoots
Plant regeneration
