摘要
目的探讨应用气相色谱技术分析全细胞脂肪酸进行分枝杆菌菌种鉴定方法的准确性和实用性。方法应用美国MIDI公司开发的基于细胞脂肪酸成分鉴定细菌菌种的软件系统MIS4.0,对14株分枝杆菌参考菌株和727株分枝杆菌临床分离株进行菌种鉴定,同时采用传统方法对相同菌株进行菌种鉴定,比较两种方法的检测结果。结果(1)参考菌株鉴定:应用传统方法对所有菌株均获得正确鉴定结果,应用脂肪酸分析方法则除母牛分枝杆菌外,其他13株均获得正确鉴定结果。(2)临床分离株鉴定:传统方法鉴定为结核分枝杆菌、牛分枝杆菌的625株中,应用脂肪酸分析方法有45株鉴定为非结核分枝杆菌,对结核分枝杆菌鉴定的正确率为93%;对传统方法鉴定为非结核分枝杆菌的102株菌株,脂肪酸分析法亦全部鉴定为非结核分枝杆菌,但其中7株的菌种鉴定结果不同,其鉴定正确率为93%。(3)脂肪酸分析法对结核分枝杆菌鉴定错误者主要是将其鉴定为胃分枝杆菌、次要分枝杆菌和耻垢分枝杆菌,对非结核分枝杆菌则主要是将瘰疬分枝杆菌鉴定为戈登分枝杆菌。结论应用气相色谱技术分析全细胞脂肪酸进行分枝杆菌菌种鉴定,和传统鉴定方法结果具有良好的一致性,且可通过一次性实验操作将临床中常见的分枝杆菌鉴定到种,是一种准确度高、实用性强的分枝杆菌菌种鉴定方法。
Objective To evaluate the accuracy and applicability of species identification method of Mycobacteria by applying gas chromatography analysis of whole-cell fatty acid. Methods Species identification of 14 reference strains and 727 clinical isolates of Mycobacteria were performed by using MIDI Sherlock Microbial Identification System (MIS)4.0 based on gas chromatography analysis of whole-cell fatty acid, and the results were compared with those of conventional species identification method. Results (1) By using the conventional method, all 14 reference strains were identified correctly. Except for Mycobacterium.vaccae, the result obtained by MIS were identical to that of conventional method. (2) Among 625 clinical isolates identified as Mycobacterium tuberculosis (MTB)and Mycobacterium bovis, 45 strains were mistakenly identified as Nontuberculous Mycobacteria (NTM) by MIS. Its accuracy was 93%. For 102 NTM strains, results of group identification showed no difference between the two methods. However, 7 results of species identification were not consistent ,the accuracy being 93%. (3) MIS mainly mistook MTB for Mycobacterium gastri,Mycobacterium triviale and Mycobacterium smegmatis, and Mycobacterium scrofulaceum for Mycobacterium gordnae among NTM. Conclusions The results of species identification of Mycobacteria by applying gas chromatography analysis of whole-cell fatty acid are in accordance with those of conventional method for the majority of the strains, and MIS can identify Mycobacteria to species level by a single experiment.It can be considered as a good method for species identification of Mycobacteria in terms of its accuracy and applicability.
出处
《中华结核和呼吸杂志》
CAS
CSCD
北大核心
2005年第6期403-406,共4页
Chinese Journal of Tuberculosis and Respiratory Diseases
基金
广东省卫生厅立项课题基金资助项目(A2004566)
