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两种不同细菌所致急性中耳炎对内耳损伤的差异 被引量:3

The difference of inner ear ultrastructure in acute otitis media induced by different virulent bacteria
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摘要 目的研究两种毒力不同的细菌导致的急性中耳炎对内耳超微结构损伤的差异。方法豚鼠麻醉后,一组在无菌条件下于右耳经鼓膜注射1×10·8L-1的绿脓杆菌液100μL,左耳作正常对照;另一组同法注射金葡菌液。三天后常规处理,透射、扫描电镜观察。结果造模后三天,绿脓杆菌感染组及金葡菌感染组基底膜、血管纹均可见细胞损伤、细胞器变性,绿脓杆菌感染组内耳超微结构的改变明显重于金葡菌感染组。结论急性中耳炎早期即可有内耳的形态学改变,绿脓杆菌引起的急性化脓性中耳炎可能会导致严重感音神经性聋。 Objective To investigate the difference of inner ear ultrastructure in acute otitis media induced by different virulent bacteria. Methods Sixteen guinea pigs were randomly divided into two groups. The right middle ears were inoculated with 0.1ml 1×108/ml staphyloccoccus aureus or pseudomonas aeruginosa respectively. The left ear serves as control. On the 3rd day after infection, alteration of the cochlea was observed by scaning and transmisson electron microscopy. Results On the 3rd day after infection, the damage of cells and degeneration of organelles could be observed in both groups of staphyloccoccus aureus and pseudomonas aeruginosa. The alterations of ultrastructure in the pseudomonas aeruginosa-infected group are more apparent than those in the staphyloccoccus aureus-infected group. Conclusion There are morphological changes of the inner ear in early acute otitis media. The acute otitis media infected by pseudomonas aeruginosa may induce serious sensorineural hearing loss.
出处 《中华耳科学杂志》 CSCD 2005年第2期128-131,共4页 Chinese Journal of Otology
基金 国家自然科学基金资助项目(30371528)
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  • 1[1]Papp Z, Rezes S, Jokay I, et al. Sensorineural hearing loss in chronic otitis media. Otol Neurotol, 2003, 24:141-144.
  • 2[2]Cusimano F, Cocita V C, D'Amico A. Sensorineural hearing loss in chronic otitis media. J Laryngol Otol,1989,103:158-163.
  • 3[3]Levine B A,Shelton C, Berliner K I,et al. Sensorineural loss in chronic otitis media. Is it chinically significant? Arch Otolaryngol Head Neck Surg, 1989, 115:814-816.
  • 4[4]MacAndie C, O'Reilly B F. Sensorineural hearing loss in chronic otitis media. Clin Otolaryngol, 1999,24:220-222.
  • 5[5]Kawauchi K,DeMaria T F,Lim D J. Endotoxin permeability through the round window. Acta Otolaryngol Suppl( Stockh), 1988,457 : 100-115.
  • 6[6]Engel F, Blatz R, Kellner J, et al. Breakdown of the round window membrane permeability barrier evoked by Streptolysin O: possible etiologic role indevelopment of sensorineural hearing loss in acute otitis media. Infect Immun, 1995,63: 1305- 1310.
  • 7[7]Paparella M M,Morezono T,Le C T,et al. Sensorineural hearing loss in otitis media. Ann Otol Rhinol Laryngol, 1984,93: 623 - 629.
  • 8Shi Z R,J Histochem Cytochem,1988年,36卷,317页
  • 9赵富玺,医学微生物学,1999年,90页
  • 10刘玉怀,北京医学,1998年,20卷,4期,267页

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  • 1Ooi MH, Solomon T. Evaluation of different clinical sample types in diagnosis of human enterovirus 71-assoclated hand-foot and mouth disease[J].J Clin Microbiol, 2007,45(6) : 1858.
  • 2Mosnier, I.,et al.Langerhans" cell histiocytosis of the labyrinth inadults. Otol Neurotol, 2004,25(1): 27-32.
  • 3Okano, T.,T. Nakagawa,J. Ito.Distribution of bone marrow-derivedcells in the vestibular end organs and the endolymphatic sac. ActaOto-Laryngologica, 2010, 130:88-94.
  • 4Forseni, M.,et al.Infiltration of immunocompetent cells in the mid-dle ear during acute otitis media: a temporal study. Am J Otol, 1999,20(2):152-157.
  • 5Harris, J.P., et al.Immunopathology of the inner ear: an update. AnnN Y Acad Sci, 1997,830: i66-178.
  • 6Harris, J.P,A.F. Ryan.Fundamental immune mechanisms of the brainand inner ear. Otolaryngol Head Neck Surg, 1995,112(6):639-653.
  • 7Ma, C., et al. Characterization of an experimentally induced innerear immune response. Laryngoscope, 2000, 110: 451—456.
  • 8Ryan, A.F., et al. Immunological damage to the inner ear: currentand future therapeutic strategies. Adv Otorhinolaryngol, 2002, 59:66-74.
  • 9Gloddek, B., K. Lamm, W. Arnold. Pharmacological influence on in-ner ear endothelial cells in relation to the pathogenesis of sensori-neural hearing loss. Adv Otorhinolaryngol, 2002,59: 75-83.
  • 10Ryan, A.F., J.P. Harris, E.M. Keithley. Immune-mediated hearingloss: basic mechanisms and options for therapy. Acta OtolaryngolSuppl, 2002,(548): 38-43.

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