摘要
为了观察骨髓间充质干细胞(BMMSC)对异基因骨髓移植(alloBMT)后急性移植物抗宿主病(aGVHD)和移植物抗白血病(GVL)效应的影响,以急性淋巴细胞白血病(ALL)小鼠为动物模型,对其进行alloBMT的同时,静脉输注体外培养的供鼠BMMSC,同时设立单纯alloBMT对照组。记录受鼠存活时间;观察受鼠发生aGVHD一般反应及病理学变化;用流式细胞术检测受鼠CD4+、CD8+T细胞亚群的差异;采用性染色体检查检测嵌合状况。结果显示:BMMSC推迟aGVHD发生的时间;降低CD4+T细胞的同时增加CD8+T细胞的数量;明显延长受鼠alloBMT后的生存时间。结论:BMMSC与alloBMT联合进行具有一定的GVL效应;BMMSC能抑制alloBMT后aGVHD的发生,但同时具有一定的GVL效应。
To investigate the effect of bone marrow mesenchymal stem cells (BMMSC) on acute graft versus host disease (aGVHD) and graft versus leukemia (GVL) after allogeneic bone marrow transplantation (allo-BMT), both bone marrow cells and BMMSC obtained after three to four weeks of culture from donor mice were transplanted into the recipient mice injected with acute lymphocytic leukemia cells 5 days before, the control group was injected with bone marrow cells alone. The survial time after allo-BMT was recorded; the general manifestation and pathological changes of aGVHD in recipient mice were observed; the effects of BMMSC on the quatity of CD4+ and CD8+T cell in vivo after allo-BMT were evaluated by flow cytometry; chimerism was detected by sex chromosome. The results showed BMMSC could increase obviously the survival time, and delay onset of aGVHD, BMMSC could decrease the amount of CD4+T cell and increase CD8+T cell in vivo. It is concluded that cotransplantation of bone marrow cells with BMMSC from the same donor mice has GVL effect. BMMSC can alleviate aGVHD and maintain GVL effect after allo-BMT.
出处
《中国实验血液学杂志》
CAS
CSCD
2005年第3期404-407,共4页
Journal of Experimental Hematology
关键词
骨髓间充质干细胞
异基因骨髓移植
急性移植物抗宿主病
移植物抗白血病
bone marrow mesenchymal stem cell
allogeneic bone marrow transplantation
acute graft versus host disease
graft versus leukemia
