摘要
目的 探讨腺苷(ADO)对大鼠移植胰腺再灌注损伤的保护作用。方法 制备同系大鼠异位全胰十二指肠移植模型。大鼠移植术前和术后5min分别静脉注射药物ADO, 8 PT( 1, 3二甲基8 苯基黄嘌呤)和ADO+ 8 PT,然后将实验分为6组: ( 1 )空白对照组(C组); ( 2 )移植对照组; ( 3 )移植+ADO组(ADO); ( 4 )移植+ 8 PT组( 8 PT); ( 5 )移植+ADO+ 8 PT组(ADO+ 8 PT ); ( 6 )移植+ [α32P]ADO(放射自显影组)。移植2 4h后检测胰腺组织中三磷酸腺苷(ATP)和总腺苷核苷酸(TAN)水平,检测血糖、血清脂肪酶、淀粉酶,测定移植胰腺组织中髓过氧化酶(MPO)活性,并进行组织学观察;放射自显影组鉴定胰腺细胞内ADO含量。结果 胰腺组织ATP和TAN水平:ADO组>移植对照组>ADO+ 8 PT组> 8 PT组(均为P < 0. 0 5 );移植后2 4h血糖、血清脂肪酶和胰腺组织中MPO水平:ADO组<移植对照组<ADO+ 8 PT组< 8 PT组(均为P < 0. 0 5 );ADO组保存的胰腺组织结构损伤明显轻于其他移植组;放射自显影显示外源性[α32 P]ADO进入胰腺细胞内。结论 外源性应用ADO或8 PT能分别增加或减少移植后胰腺组织ATP和TAN含量;腺苷对移植胰腺再灌注损伤有保护作用。
Objective To study the protective effects of adenosine(ADO) on reperfusion injury following pancreaticoduodenal transplantation in rats. Methods The homologous male Wistar rat model of heterotopic total pancreaticoduodenal transplantation was made. The rats received IV injection of either ADO,8-PT(1, 3-dimethyl 8-phenylxanthine) or ADO+8PT at 5 min before or 5 min after operation,and then were divided into 6 groups:(1)blank control(C group);(2)transplant control group;(3)transplant +ADO group(ADO);(4)transplant +8-PT group(8-PT);(5)transplant+ADO+8-PT group(ADO+8-PT),and (6) transplant+[α- 32 P] ADO(radiation-visible group).AT 24h after transplantation,the pancreatic tissue concentration of adenosine triphosphate(ATP) and total adenine nucleotides (TAN) were determined, blood sugar (BG) and serum concentration of amylase and lipase were examined, the activity of myeloperoxidase (MPO) in pancreatic graft tissue was measured, and histological observation was made. [α- 32 P]ADO was used to radiologically identify the content of ADO in pancreatic tissue.Results Pancreatic tissue concentration of ATP and TAN[(5.72±0.47)μmol/g, (8.71±0.91)μmol/g]in ADO group> transplant control group[(3.39±0.58)μmol/g, (5.01±0.78)μmol/g]>(ADO+8-PT)group[(2.92±0.24)μmol/g, (4.84±0.41)μmol/g]>8-PT group[(2.05±0.66)μmol/g, ( 3.98 ±0.41)μmol/g] 24h after transplantation(P<0.05).The value of BG and serum lipase and MPO in ADO group< transplant control group < (ADO+8-PT)group < 8-PT group(P<0.05). Lightmicroscopic studies showed that histomorphological changes of pancreas in experimental group were muchless than that in other groups.Radioactive [α- 32 P]ADO was found that the radiactive meteria can enter the pancreatic acinar cells.Conclusions Exogenous ADO can increase the amount of ATP and TAN in pancreatic graft tissue,while 8-PT reduces it. ADO has protective effects on the ischemia/ reperfusion injury of pancreaticoduodenal graft.
出处
《中国普通外科杂志》
CAS
CSCD
2005年第5期351-354,共4页
China Journal of General Surgery
基金
辽宁省科委重大资助项目(00225001)
关键词
腺苷/治疗应用
胰腺移植
再灌注损伤
Adenosine/ther use
Pancreas Transplantion
Reperfusion Injury
