摘要
利用PCR RFLP技术(限制性内切酶采用HinfⅠ和HaeⅢ)检测了培育品种77头中畜黑猪I系的心肌脂肪酸结合蛋白(H FABP)基因座位的遗传变异,同时对H FABP的不同PCR RFLP基因型对生产性能的影响作了初步统计分析,旨在探讨利用H FABP进行肉质性状辅助选择,是否对生产性能产生不利影响。结果表明,在H FABP基因5′上游和内含子2 区段,均发现HinfⅠRFLP 和HaeⅢRFLP、HinfⅠ* RFLP;结果显示,在HinfⅠRFLP位点上,HH和hh 2种基因型间个体170日龄体重差异显著(P<0.05),除此之外,未发现其它酶切位点的不同基因型个体生长性能之间的显著差异。研究结果为今后开展猪肉质性状和生产性能的分子辅助选育奠定应用基础。
In this study, the genetic variation of porcine heart fatty acid-binding protein(H-FABP) gene was detected by PCR-RFLP with HinfⅠ and HaeⅢ in 77 pigs of Zhongxu Black Line I. Meanwhile, effects of different PCR-RFLP genotypes of H-FABP gene on main growth traits were analyzed in order to investigate whether they have unfavorable effects on growth traits or not during making meat qualities assistant selection by H-FABP gene. The results showed three variant restriction sites of porcine H-FABP gene including HinfⅠ-RFLP in 5′-upstream, HaeⅢ-RFLP and HinfⅠ *-RFLP in intron 2, were conformed by PCR-RFLP method. Statistic significant difference(P<0.05) of product performance (weight of 170 days) was found among animals grouped by different genotype at the HinfⅠ-RFLP site, while groups divided by any other genotypes had no significant differences among all product performance. Conclusion made in this paper will contribute to molecular assistant selection of economic traits including meat quality and production performance in pig breeding.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2005年第6期555-558,共4页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
国家"十五"科技攻关项目(2004BA514A 13)
"863"项目(2003AA243030)
