期刊文献+

用荧光半定量RT-PCR检测蚊溴氰菊酯抗性 被引量:3

Detection of deltamethrin resistance in Culex pipiens pallen with semi-quantitative fluorescent RT-PCR assay
下载PDF
导出
摘要 目的:建立蚊对菊酯类杀虫剂抗性的PCR检测方法。方法:应用荧光半定量RT鄄PCR,检测经SSH结合cDNA芯片分离鉴定的淡色库蚊对溴氰菊酯抗性相关基因NYD鄄Tr在抗性品系与敏感品系中的表达水平。结果:经荧光半定量RT鄄PCR检测,NYD鄄Tr进入指数期的循环数抗性品系早于敏感品系,抗性品系组比敏感品系组先进入指数增长期。结论:以NYD鄄Tr作为靶基因,荧光半定量RT鄄PCR可区分淡色库蚊对溴氰菊酯抗性品系与敏感品系,亦即可检测淡色库蚊溴氰菊酯抗性。 Objective: To establish a semi-quantitative fluorescent RT-PCR assay for detection of deltamethrin resistance in mosquitoes. Methods: Semi-quantitative fluorescent RT-PCR were used to detect the expression level of NYD-Tr gene which was associated with deltamethrin resistance in Culex pipiens pallens. Results: RT-PCR assays showed that the relative expression levels of NYD-Tr mRNA were higher in deltamethrin resistant strains than in susceptible strains of Cx.pipiens pallens. Conclusion: Results of this study demonstrate that semi-quantitative fluorescent RT-PCR analysis is probably an effective tool for detecting deltamethrin resistance in Cx.pipiens pallens.
出处 《南京医科大学学报(自然科学版)》 CAS CSCD 北大核心 2005年第7期447-449,共3页 Journal of Nanjing Medical University(Natural Sciences)
基金 国家自然科学基金资助项目(30170835) 南京师范大学生命科学学院江苏省资源生物技术重点实验室开放研究课题资助项目(164070300801)
关键词 淡色库蚊 抗药性 溴氰菊酯 荧光半定量RT-PCR culex pipiens pallens resistance deltamethrin semi-quantitative fluorescent RT-PCR
  • 相关文献

参考文献11

  • 1Hirst SI, Stapley LA. Parasitology: the dawn of a new millennium[J]. Parasitol Today,2000,16(1):1-3.
  • 2Hemingway J, Ranson H. Insecticide resistance in insect vectors of human disease [J]. Annu Rev Entomol,2000,45:371-391.
  • 3Rust MK,Waggoner M,Hinkle NC,et al.Development of a Larval Bioassay for Susceptibility of Cat Fleas (Siphonaptera:Pulicidae) to Imidacloprid [J]. J Med Entomol, 2002,39(4): 671-674.
  • 4WU HW,TIAN HS, WU GL,et al.Culex pipiens pallens:identification of genes differentially expressed in deltamethrin-resistant and-susceptible strains [J]. Pestic Biochem Physiol,2004,79:75-83.
  • 5LI XL, SUN LX, MA L, et al. Biotic characteristics in the of deltamethrin-susceptible and resistant strains of Culex pipiens pallens (Diptera:Culicidae) in China [J]. Appl Entomol Zool, 2002,37:305-308.
  • 6Pfaffl M. Development and validation of an enternally standardized quantitative insulin-like growth factor-1 RTPCR using LightCycler SYBR Green Ⅰ technology [J].Biochemica,2000,3:13-16.
  • 7Vandesompele J, De Paepe A, Speleman F. Elimination of Primer-Dimer Artifacts and Genomic Coamplification Using a Two-Step SYBR Green Ⅰ Real-Time RT-PCR [J].Anal Biochem ,2002,303:95-98.
  • 8Pfaffl MW, Horgan GW, Dempfle LR. Elative expression software tool (REST) for group-wise comparison and statistical analysis of relative expression results in real-time PCR[J]. Nucleic Acids Res,2002,30:36.
  • 9Das H, Koizumi T, Sugimoto T, et al. Quantitation of Fas and Fas ligand gene expression in human ovarian, cervical and endometrial carcinomas using real-time quantitative RT-PCR[J]. Br J Cancer,2000,82:1682-1688.
  • 10刘宝芳,席志宾.各型乙型肝炎患者血液HBV DNA水平与临床的关系[J].中华肝脏病杂志,2002,10(1):65-66. 被引量:56

共引文献55

同被引文献91

引证文献3

二级引证文献19

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部