摘要
[目的]从胃肠激素水平,研究疏肝活血健脾方对门脉高压性胃病(PHG)的防治作用。[方法]选择健康雄性Wistar大鼠120只,随机分为8组,采用一期门静脉缩窄法制备PHG动物模型。按要求造模灌胃后,在预定时间采胃组织,用免疫组织化学技术图像分析法检测胃底组织促胃液素(GAS)、血管活性肠肽(VIP)水平。[结果]A组大鼠胃底组织GAS、VIP免疫组化平均灰度值较F组升高(P<0.01);B、C、D组大鼠胃底组织GAS、VIP免疫组化平均灰度值较E组升高(P<0.01),B组疗效优于D组(P<0.05)。[结论]GAS、VIP在PHG的发病机制中起着重要作用。疏肝活血健脾方对PHG的防治效果肯定。
Objective]To explore the preventive and curative effect on portal hypertensive gastropathy (PHG) of Shugan Huoxue Jianpi formula (SHJF) which can smooth liver-qi, acting blood and strengthen spleen.[Methods] One hundred and twenty Wistar male rats were divided into eight groups randomly. Portal hypertensive gastropathy (PHG) model was made by the method of partial portal vein ligation (PVL). Then vasoactive intestinal peptide (VIP) and gastrin (GAS) of each group were tested with immunohistochemical method.[Results] The average B- mode tite of GAS, VIP in gastric fundus tissue of group A was significantly higher than that of group F (P<0.01). The average B-mode tite of GAS, VIP of group B, C, D in gastric fundus tissue was significantly higher than that of group E (P<0.01). The effect of group B was better than that of group D(P<0.05).[Conclusion]GAS and VIP have close relationship with the principle of PHG. SHJF has positive effects in preventing and curing PHG.
出处
《中国中西医结合消化杂志》
CAS
2005年第3期164-167,共4页
Chinese Journal of Integrated Traditional and Western Medicine on Digestion
关键词
胃疾病
门脉高压性
促胃液素
血管活性肠肽
疏肝活血健脾方
stomach disease,portal hypertensive
gastrin
vasoactive intestinal peptide
Shugan Huoxue Jianpi formula (a prescription of smoothing liver-Qi, acting blood and strengthening spleen)
