摘要
从不同污泥与堆煤土样中分离出七株菌株,均能专一地切断二苯并噻吩(DBT)中的C-S键,沿4S途径代谢,生成2-羟基联苯(2-HBP)。通过对七株菌株46h休止细胞和繁殖期生长细胞脱硫活性的比较,筛选出具有较高脱硫活力的微杆菌(Microbacteriumsp.)ZD-M2和棒杆菌(Corynebacteriumsp.)ZD-C7,并在生长温度30℃,pH7.0,0.5mmol?L?1DBT作为唯一硫源,以甘油为碳源,用摇瓶培养法测定了它们的生长和脱硫曲线。以正十六烷为模型油相,在油水体积比为1.0的两相介质中考察两菌株的脱硫特性。结果表明,有机相的存在增加了菌株的脱硫活性,但活性持续时间只有9小时较水溶液介质中的短。同时两菌都能脱除燃料油中的其他含硫杂环化合物:噻吩、苯并噻吩、二苯硫醚和4,6-二甲基二苯并噻吩的硫,是有工业化应用前景的生物脱硫催化剂。
Seven bacterial strains capable of converting dibenzothiophene into 2-hydroxybiphenyl by cleaving the carbon-sulfur bonds were isolated from sludge and soil samples. Among them two strains, identified as Microbacterium sp.ZD-M2 and Corynebacterium sp.ZD-C7, were chosen for further characterization because of their relatively high desulfurization activities. Their growth curves were investigated under the condition of 30°C, pH 7.0, with 0.5 mmol·L-1DBT as the sole sulfur-source and glycerol as carbon nutrient in a shaking bottle. Desulfurization ability was studied in a two phase medium, in which the hexadecane was used as model oil and the volume ratio of oil to water is 1.0. The results show that the presence of hexadecane enhances the activity of desulfurization, however the enzymes are inactivated after 9 hours. The further experiments show that other sulfur heterocyclics, such as thiophene, benzothiophene, diphenylsulfid and 4,6-dimenthyldibenzothiophene, can also be degraded by these two strains.
出处
《高校化学工程学报》
EI
CAS
CSCD
北大核心
2005年第3期392-397,共6页
Journal of Chemical Engineering of Chinese Universities
基金
国家自然科学基金(20276068)
关键词
含硫杂环化合物
生物脱硫
分离
菌株
Bacteria
Carbon
Characterization
Chemical bonds
Degradation
Desulfurization
