摘要
目的同时测定10批栀子药材中栀子苷和西红花苷-1的含量。方法采用RP-HPLC法,ShimpackVP-ODS柱,乙腈-水梯度洗脱,检测波长238nm(0～20min,检测环烯醚萜苷类)、440nm(20～60min,检测西红花苷类)。结果和结论该方法简便、准确、重复性好,可同时测定栀子中不同检测波长的两大类有效成分。
OBJECTIVE To simultaneously determine the contents of geniposide and crocin-1 Fructus Gardeniae. METHODS RP-HPLC method was adopted.Shimpack VP-ODS column, gradient elution using acetonitrile-water as mobile phase, and detective wavelength of 238 nm(0-20 min,to detect iridoid glycosides), 440 nm(20-60 min,to detect crocins). RESULTS AND CONCLUSION This method simultaneously determined the two main components at different detective wavelength in Fructus Gardeniae.It is a simple,accurate and credible qulity control method for Fructus Gardeniae.
出处
《华西药学杂志》
CAS
CSCD
北大核心
2005年第3期223-225,共3页
West China Journal of Pharmaceutical Sciences
