摘要
给SD大鼠股四头肌注射带有增强型绿色荧光蛋白(EGFP)基因的质粒pEGS/2SS,每只100μg。注射后不同时间剖杀,取注射部位肌肉、心、脑、垂体、肝、脾、肾、小肠、胃、子宫、卵巢作冰冻切片,于荧光显微镜下检查。发现pEGS/2SS仅在注射部位肌肉表达,72h表达最强,10d后明显减弱,2周后消失。其他组织及对照组未检测到绿色荧光。表明pEGS/2SS在体内表达、分布方面具有较好的安全性。
The SD rats were killed at different time after injection of 100 μg plasmid pEGS/2SS containing enhance green fluorescent protein(EGFP)via quadriceps femoris to harvest tissues and organs of their muscle(located at place of injection),heart,brain,pituitary gland,liver,spleen,kidney,small intestine,stomach,uterus and ovary.The tissue slices were detected by fluorescent microscope.The plasmid pEGS/2SS expression was observed only in tissue of injected muscle.The fluorescent was strongest about 72 h after injection.It weakened significantly in 10 days and disappeared in 2 weeks.There are no fluorescent detected in other tissues and in the animals of control groups.In conclusion,pEGS/2SS is safe on expression and tissue distribution in rat.
出处
《南京农业大学学报》
CAS
CSCD
北大核心
2005年第2期137-139,共3页
Journal of Nanjing Agricultural University
基金
国家自然科学基金项目(30270959)
上海市科技兴农重点攻关项目(农科攻字(2002)第136号)
关键词
生长抑素
DNA疫苗
安全性
绿色荧光蛋白
somatostatin
DNA vaccine
safety
enhance green fluorescent protein(EGFP)
