摘要
通过提取水稻叶片基因组DNA,设计PCR扩增特异引物,克隆单子叶特异表达启动子Ac-tin1基因,与T载体连接,转化E.coliDH5α,得到重组子,经酶切和序列分析鉴定,该片段分子大小为1238bp,通过序列对比分析发现与已发表的Actin1碱基序列有99.60%的同源性。特异启动子基因驱动的抗真菌肽(AFP)基因植物表达载体,就可以进行早熟禾黑麦草等单子叶牧草的遗传转化,为单子叶牧草和草坪草的抗真菌病研究提供方法。
Rice (Oryza sativa) genomic DNA was extracted from leaves. A 1238 bp monocotyledon express promoter Actin1 was cloned by PCR using artificial synthetic special primers and combined with plasmid pUCm-T easy vector and transformed E. coli DH 5α Sequence analysis indicated that the homology between this fragment and the reported sequence was 99.60%. This work was a foundation for exploring grass anti-fungal transformation.
出处
《草原与草坪》
CAS
2005年第3期66-68,71,共4页
Grassland and Turf
基金
甘肃亚盛集团
关键词
克隆
启动子
植物表达载体
cloning
promoter
plant expression vector
