摘要
目的研究局部脑缺血再灌注后N-甲基-D-天冬氨酸(NMDA)受体I型亚单位(NR1)mRNA表达与细胞凋亡的关系及银杏叶制剂(Egb)对其影响。方法140只SD大鼠被随机分成假手术组、缺血再灌组,即MCAO组和Egb治疗组。每组大鼠再随机分成4组:分别在再灌注后6,24,48,96 h处死。进行TUNEL染色检测缺血区细胞凋亡情况,并用原位杂交方法检测NR1 mRNA。结果MCAO组大鼠大脑皮质梗死灶的凋亡细胞和NR1mRNA表达与假手术组比较明显增强(P<0.01),而Egb治疗组这两项指标的表达明显低于MCAO组(P<0.01)。结论再灌注后脑组织中NR1mRNA表达增强,与细胞凋亡密切相关。银杏叶制剂可以明显抑制NR1mRNA的表达,减轻细胞凋亡。
Objective To study the relationship between expression of NMDA receptor 1(NR1)mRNA and neuron apoptosis in rats during focal cerebral ischemia-reperfusion(I/R),the effect of Ginkgo biloba extract(Egb) on them and related mechanism. Methods One hundred and forty SD rats were randomized into 3 groups: sham-operated group (groupⅠ, n=20), ischemia-reperfusion group (MCAO group/groupⅡ,n=60) and Egb treatment group (groupⅢ, n=60). The rats were killed at 6, 24, 48, 96 h after reperfusion for TUNEL and in situ hybridization analysis. TUNEL was used to show cell apoptosis. Results Compared with groupⅠ, the expression of NR1 mRNA and the number of apoptosis cells in I/R brain tissue increased significantly in groupⅡ. Compared with groupⅡ, NR1 mRNA expression and cell apoptosis decreased obviously in groupⅢ. Conclusions The expression of NR1 mRNA in I/R brain tissue increased significantly than that in groupⅠ and might be closely related to neuron apoptosis. And Egb can inhibit the expression of NR1 mRNA in ischemic areas and the neuron apoptosis subsequently.
出处
《神经科学通报》
CSCD
2005年第3期210-214,共5页
Neuroscience Bulletin
