摘要
目的探讨不同细胞因子组合对脐带血中ACl33+细胞表面趋化因子受体(CXCR4)、粘附分子VLA4(CD49d)、LFA1(CD11a)和Lselectin(CD62L)表达的影响。方法采用直接免疫荧光标记法,使用流式细胞仪测定新鲜分离的、不同培养条件下体外培养7d、14d的脐带血中ACl33+细胞表面CXCR4和CD49d、CD11a和CD62L的表达情况。结果新鲜脐带血ACl33+细胞中CXCR4的表达率为(50.2±12.5)%;CD49d的表达率为(53.2±15.6)%,CD11a的表达率为(98.1±2.4)%,CD62L的表达率为(52.4±16.6)%。在无血清、无细胞因子存在的条件下,体外培养第7d及14d,CXCR4、VLA4、CD11a和CD62L的表达显著下降(P<0.05)。在有细胞因子存在的条件下,短期液体扩增培养后,脐带血ACl33+细胞中CXCR4、VLA4、CD11a和CD62L的表达率较新鲜ACl33+细胞均有不同程度的增高(P<0.05);IL3+FL+SCF组较IL11+FL3+SCF组表达率高,其中以培养第14dCXCR4、CD62L的表达增高最明显。结论在体外短期扩增培养过程中,早期效应细胞因子能显著上调脐带血ACl33+细胞CXCR4、CD49d、CD11a和CD62L等的表达;用SCF、FL和IL3组合扩增脐带血,不仅能获得足够数量的造血干/祖细胞,还能增加造血干/祖细胞的迁移和归巢能力。
Objective To investigate the effect of different cytokine combinations on the expression of chemokine receptor(CXCR4), VLA4(CD49 d), LFA-1(CD11a) and L-selectin (CD62L) in umbilical cord blood AC133^+ cells.Methods The expression rate of the CXCR4, CD49 d, CD11a and CD62L on the surface of umbilical cord blood AC133^+ cells which were freshly isolated and incubated in serum-free liquid culture system for 7 d and 17 d with different cytokine combinations in vitro were analyzed by flow cytometric two-colour direct immunoflurescence methods. Results The expression rate of the CXCR4, CD49 d, CD11a and CD62L on the surface of fresh umbilical cord blood AC133^+ cells was 50.2%±12.5%, 53.2%±15.6%, 98.1%±2.4 SED and 52.4%±16.6 SED respectively. After short-term incubation in serum-free culture system with no cytokine in vitro at d 7 and d 14, the expression rate remarkably declined. After short-term incubation in serum-free culture system with cytokines in vitro, the expression rate was higher at different levels than that in fresh umbilical cord blood. The expression rate was remarkably higher in the presence of IL-3, FL and SCF combinations than that in the presence of IL-11, FL and SCF combinations. [WTHZ]Conclusions [WTBZ]During short-term cul- ture in vitro, the early-effective cytokines can up-regulate the expression of CXCR4, CD49d, CD11a and CD62L in umbilical cord blood AC133^+ cells. In the presence of IL-3, FL and SCF combinations, we may not only acquire enough hematopoietics stem/progenitor cells, but also gain its migration and homing capabilities.
出处
《苏州大学学报（医学版）》
CAS
北大核心
2005年第3期351-354,共4页
Suzhou University Journal of Medical Science
基金
国防科工委"十五"国防预研项目资助课题(Y-020403-QS)
