摘要
目的检查急性脊髓损伤(SCI)后细胞凋亡及CD95的表达,初步探讨白细胞介素10(IL10)对大鼠SCI后细胞凋亡的干预作用。方法将SD雄性大鼠48只随机分为假手术组(n=6)、损伤组(n=30)、治疗组(n=12)。使用改良Allen法制作脊髓损伤模型,分别手术后4h、8h、24h、72h及7d处死并取材(每时间点n=6)。治疗组损伤后30min给予IL10。应用HE染色、免疫组化及凋亡细胞原位末端标记法(TUNEL)对损伤脊髓组织进行检测。结果损伤后4h,损伤段灰质可见CD95及TUNEL阳性细胞。CD95阳性细胞表达于8h达高峰;TUNEL阳性细胞于24～72h达高峰。TUNEL阳性细胞主要见于神经元及胶质细胞,以胶质细胞为主。IL10治疗组的TUNEL阳性细胞数在8h、72h明显减少;免疫组化检测CD95表达降低。正常组未见CD95及TUNEL阳性细胞表达。结论SCI后CD95的表达可能在SCI后诱导细胞凋亡的过程中起重要作用;早期应用IL10能干预SCI后的细胞凋亡,但不减少CD95的表达。
Objective To examine the cellular distribution of apoptosis and expresson of CD95 following T_8～T_9 spinal cord injury and whether IL-10 may reduce apoptosis. Methods The spinal cord injury of the SD rats were induced with Allen's way by a 10 g×2.5 cm impact on the posterior T_8～T_9 spinal cord. Rats were sacrificedat the 4 th,8 th,24 th,72 th hour and 7 th day after injury (n=6 for each time point). IL-10 was administered the 30 th minute after SCI. Three segments of each spinal cord were cut for morphological studies, including hematoxylin and eosin staining, immunohistochemical staining and the terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) methods. Results CD95 immunoreactivity was present in gray matter of the injured region after 4 h, with a maximum presence the 8th hour gray matter. The TUNEL-positive cells were found in the injuried 4 hours later, with a maximum presence the 24th hour in ent, and labeled glial cells in white matter reached a peak at the 72nd hour. The TUNEL-positive cells were neural and glial cells in gray matter, especially in the latter one. In the apoptosis of IL-10 treatment group there was distinct less than that of injury group in 8h and 72 h after SCI. Six additional normal rats did not show any CD95 and TUNEL immunoreactivity. Conclusions The high expression of CD95 may play an important role in induction of apoptosis after spinal cord injury. IL-10 can reduce the numbers of apoptotic cells, but it dose not decrease the expression of CD95.
出处
《苏州大学学报（医学版）》
CAS
北大核心
2005年第3期395-397,401,共4页
Suzhou University Journal of Medical Science
