摘要
目的探讨体外冲击波碎石术(ESWL)对肾细胞内游离钙([Ca2+]i)水平及肾细胞凋亡的影响与钙通道阻滞剂的干预作用。方法30只家兔制成单肾模型,随机等分为对照组、ESWL组和ESWL+维拉帕米预处理组。ESWL后24h获取肾脏,采用Fura-2/AM荧光指示剂测定肾细胞内[Ca2+]i浓度,原位缺口末端标记法(TUNEL)和流式细胞计数法检测凋亡细胞。结果ESWL组肾细胞内[Ca2+]i增高、肾细胞凋亡率增加(P<0.01);维拉帕米预处理组上述改变明显改善(P<0.01)。ESWL后肾细胞内[Ca2+]i增高与细胞凋亡率有相关趋势(r=0.63,P<0.05)。结论ESWL所致肾细胞内[Ca2+]i超载和肾细胞凋亡增加,可能是ESWL肾损伤的重要机制;钙通道阻滞剂有拮抗细胞内[Ca2+]i超载和肾细胞凋亡增加的作用。
Objective] To observe the effects of extracorporeal shock wave lithotripsy(ESWL)on calcium ion([Ca2+]i) level and apoptosis in renal cells of rabbit and the protective effects of pretreatment with calcium channel blocker on these varieties. [Methods] Thirty healthy male domestic rabbits were surgically managed to the mono-nephron models and randomized into 3 groups consisting of ten each: control group, ESWL group and ESWL plus verapamil pretreatment group. The kidneys from rabbits were obtained 24 hours after ESWL. Renal intracellular [Ca2+]i level was measured by Fura-2/AM fluorescence assay, cell apoptosis was detected by TUNEL(TdT-mediated dUTP Nick End Labelling) and flow cytometry at the same time. [Results] The intracellular [Ca2+]i levels and apoptotic rates increased significantly in ESWL group compared with those in control group (P <0.01). The changes mentioned above were alleviated obviously in ESWL plus verapamil pretreatment group. The intracellular [Ca2+]i levels and apoptotic rates were significantly correlated(r=0.63, P<0.05). [Conclusions] Renal intracellular [Ca2+]i overloading and cell apoptosis increasing may have an important role in the pathologic process of renal injuries induced by ESWL. Calcium channel blocker can hinder these changes.
出处
《中国内镜杂志》
CSCD
北大核心
2005年第5期457-459,462,共4页
China Journal of Endoscopy
基金
广东省医学科学基金资助项目(A2003246)
关键词
体外冲击波碎石术
肾脏细胞
细胞内游离钙
细胞凋亡
extracorporeal shock wave lithotripsy
intracellular calcium ion
renal cell
apoptosis
