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锂对原代培养的猪甲状腺细胞生长的影响

Effect of Lithium on the Growth of Cultured Pig Thyroid Cells
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摘要 目的 研究锂在原代培养的猪甲状腺细胞生长调节中的作用。方法 选取新鲜猪甲状腺组织,采用细胞原代培养技术和非同位素标记的细胞增殖分析法(MTT) ,检测不同浓度的氯化锂(LiCl)对甲状腺细胞生长的影响。结果 在TSH存在及不存在条件下,LiCl均呈剂量依赖性的促进甲状腺细胞的生长。结论 一定浓度的LiCl能够促进甲状腺细胞增殖。 Objective To investigate the effect of Lithium on the growth of pig thyroid gland. Methods Fresh pig thyroid cells were cultured in a monolayer system.Non-radioactive Cell Proliferation Assay(MTT) was used to detect different concentration Lithium on the growth of pig thyroid cells. Results In the condition of non-TSH and TSH,Lithium both enhance the growth of pig thyroid cells in dose-dependent manner. Conclusions Lithium can accelerate the growth of pig thyroid cells.
出处 《锦州医学院学报》 2005年第2期24-26,共3页 Journal of Jinzhou Medical College
关键词 锂元素 原代培养 动物实验 甲状腺细胞 细胞生长 Lithium thyroid cell culture growth
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参考文献6

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二级参考文献16

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