摘要
目的通过检测大肠癌组织内血管内皮生长因子(VEGF)的蛋白表达、微血管密度(MVD)及术前血清CEA值,探讨其与大肠癌病理学特征及预后的相关性。方法采用免疫组化Envision方法,对来自日本金泽医科大学一般消化器外科1990～1995年的78例手术切除大肠癌标本的癌灶、癌旁、正常大肠组织进行血管标记和染色,并进行定位观察,检测其VEGF表达及MVD计数,同时回顾分析血清CEA值变化,并与病人预后及病理特征进行比较。结果78例大肠癌病人中,48例病人(61.2%)VEGF蛋白表达阳性,MVD计数为(38.6±9.4),CEA阳性病人35例(44.7%)。它们与肿瘤浸润深度、淋巴结、血行转移及Dukes分期呈明显相关性(P<0.05)。而与肿瘤大小、组织学分型、生长方式无关(P>0.05)。而且,VEGF阳性组与阴性组5年生存率分别为66.8%、86.7%,差异显著(P<0.05);CEA阳性组与阴性组5年生存率亦有明显区别(P<0.05);VEGF+/CEA+组5年生存率最低。MVD与VEGF呈明显的相关性。结论VEGF、MVD及CEA与大肠癌浸润转移,分期及生存率呈明显相关性,VEFG和CEA联合检测,对大肠癌预后的判断更具有实际意义。
Objective To explore the correlation between the expression of vascular endothelial growth factor (VEGF),microvessel density (MVD) and CEA and pathologic feature and prognosis in colorectal carcinoma.Methods The expression of VEGF and MVD were examined by immunohistochemical staining in 78 specimens of colorectal carcinoma and CEA value of these cases were reviewed.Results The positive rate of VEGF was 61.2%.The average value of MVD was 38.6±9.4.The positive rate of CEA was 44.7%.VEGF,MVD and CEA had no relation with tumor size,histological type and growth pattern,but were significantly related with the infiltration depth,lymph node and blood metastasis and Dukes stage(P<0.05).The 5-year survival rate for group of VEFG+ and group of VEGF-was 66.8% and 86.7% respectively,the 5-year survival rate for group of CEA+ and group of CEA-being 68.6% and 90.2% respectively.The difference was significant in above two groups.The 5-year survival rate for group of VEFG+/CEA+ was 45.2%,which was significantly lower than that for group of VEGF+/CEA-,VEFG-/CEA+ and VEFG-/CEA-.Conclusion VEGF,MVD and CEA are significantly related with tumor progression and poor prognosis in colorectal carcinoma.The combining criterion of VEFG and serum CEA value should be a useful prognostic indicator and the prognosis of group of VEFG+/CEA+ is very poor for colorectal carcinoma patients.
出处
《中国实用外科杂志》
CSCD
北大核心
2005年第6期362-364,共3页
Chinese Journal of Practical Surgery
