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A Mutant Strain of a Surfactant-Producing Bacterium with Increased Emulsification Activity 被引量:4

A Mutant Strain of a Surfactant-Producing Bacterium with Increased Emulsification Activity
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摘要 As reported in this paper, a strain of oil-degrading bacterium Sp - 5 - 3 was determined to belong to Enterobacteriaceae, which would be useful for microbial enhanced oil recovery (MEOR). The aim of our study was to generate a mutant using low energy N+ beam implantation. With 10 keV of energy and 5.2× 1014 N+/cm2 of dose - the optimum condition, a mutant, S-34, was obtained, which had nearly a 5-fold higher surface and a 13-fold higher of emulsifica-tion activity than the wild type. The surface activity was measured by two methods, namely, a surface tension measuring instrument and a recording of the repulsive circle of the oil film; the emulsification activity was scaled through measuring the separating time of the oil-fermentation mixture. The metabolic acid was determined as methane by means of gas chromatography. As reported in this paper, a strain of oil-degrading bacterium Sp - 5 - 3 was determined to belong to Enterobacteriaceae, which would be useful for microbial enhanced oil recovery (MEOR). The aim of our study was to generate a mutant using low energy N+ beam implantation. With 10 keV of energy and 5.2× 1014 N+/cm2 of dose - the optimum condition, a mutant, S-34, was obtained, which had nearly a 5-fold higher surface and a 13-fold higher of emulsifica-tion activity than the wild type. The surface activity was measured by two methods, namely, a surface tension measuring instrument and a recording of the repulsive circle of the oil film; the emulsification activity was scaled through measuring the separating time of the oil-fermentation mixture. The metabolic acid was determined as methane by means of gas chromatography.
出处 《Plasma Science and Technology》 SCIE EI CAS CSCD 2005年第3期2889-2892,共4页 等离子体科学和技术(英文版)
基金 The project supported by the Knowledge Innovation Project of the Chinese Academy of Sciences(No. KSCX2-SW-324)
关键词 low energy ion beam implantation microbial enhanced oil recovery (MEOR) enterobacteriaceae sp. surface and emulsification activity gas chromatography low energy ion beam implantation, microbial enhanced oil recovery (MEOR), enterobacteriaceae sp., surface and emulsification activity, gas chromatography
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