摘要
从污泥样品中分离得到一株溴氨酸降解菌,该菌株能够以溴氨酸为唯一碳、氮源及能源生长.通过形态、生理生化特性分析以及对16SrDNA序列进行同源比较,鉴定该菌株属于鞘氨醇单胞菌属,定名为Sphingomonasxenophaga .菌株的最适降解与生长条件为:pH =7 0 ,温度3 0℃,转速15 0r·min- 1 ,接种量8% .在最佳条件下,溴氨酸的降解率可达90 %以上,菌株可耐受的溴氨酸浓度为10 0 0mg·L- 1 .通过分析菌株对水杨酸、邻苯二酚及邻苯二甲酸的利用情况,推测该菌株可能通过邻苯二酚的代谢途径降解溴氨酸.
A strain that had a capability to degrade bromoamine acid (BAA) was isolated from the sludge samples. The strain could use BAA as the only source of carbon, nitrogen and energy. It was identified as Sphingomonas xenophaga based on morphological and physio-biochemical characteristics and 16S rDNA sequence analysis. Under the optimal growth conditions, with pH of 7.0, temperature of 30℃, rotating rate of 150?r·min -1 and inoculation amount of 8%, BAA could be degraded in the high efficiency (more than 90%), and the strain could tolerate up to 1?000?mg·L -1 BAA. It was assumed that the strain might degrade BAA by the o-diphenol catabolic pathway, because it could utilize o-diphenol as sole carbon source.
出处
《环境科学学报》
CAS
CSCD
北大核心
2005年第6期785-790,共6页
Acta Scientiae Circumstantiae
基金
教育部跨世纪人才基金~~
