摘要
利用丙型肝炎病毒（ＨＣＶ）５’－端序列合成两对引物，建立了灵敏、特异的ＨＣＶＲＮＡ双扩增聚合酶链反应检测方法。用此方法及第二代Ａｂｂｏｔｔ酶联抗－ＨＣＶ检测试剂盒，检测了４４例非甲非乙型肝炎患者血清及１０名抗－ＨＣＶ阴性健康人。在４４例患者中，４１例（９３％）ＨＣＶＲＮＡ阳性，３６例（８２％）抗－ＨＣＶ阳性，３３例（７５％）ＨＣＶＲＮＡ、抗－ＨＣＶ全部阳性。３例ＨＣＶＲＮＡ阴性，但抗－ＨＣＶ阳性，另外，有８例抗－ＨＣＶ阴性，ＨＣＶＲＮＡ阳性。１０名健康人ＨＣＶＲＮＡ均为阴性。结果表明，大部分（９２％）抗－ＨＣＶ阳性患者带有ＨＣＶ，但为了检测所有病毒血症患者，抗－ＨＣＶ检测是不够的，利用双扩增ＰＣＲ方法检测ＨＣＶＲＮＡ对于抗－ＨＣＶ阴性患者的诊断是非常有用的。
A double polymerasee chain reaction, involving nested primers deduced form the 5'-noncoding region of the HCV genome, was developed for a sensitive and specific detecion of HCV RNA. With this method and Abbott seeond generation EIA kit for anti-HCV,44 paticnts with non-A,non-B hepatitis and 10 anti-HCV negative healthy subjects were tessted.Among 44 patients. HCV RNA was detected in 41(93%) and the antibody was positive in 36(82%).Thirty-three(75%)patients, were positive for both assays. The two assays gave inconsistent results in 3 patients who were negative for HCV RNA,and positive for anti-HCV,and in other 8 patients were positive for HCV FNA but negative for anti-HCV, Control sera form 10 healthy subjects ded not reveal HCV RNA. These data suggest that the majority(92%)of anti-HCV positive patients are carriers of HCV.But to the double PCR may be very useful in diagnosing anti-HCV antibody test may be insufficient, Direct detection of HCV RNA by the double PCR may be very useful in diagnosing anti-HCV negative patients.
出处
《中华实验和临床病毒学杂志》
CSCD
1994年第3期252-255,共4页
Chinese Journal of Experimental and Clinical Virology
