摘要
本研究采用双色免疫荧光染色技术，用流式细胞计观察了经Ｅ花环法分离的正常人外周血Ｔ细胞和经补体介导的细胞毒方法用ＯＫＴ８单克隆抗体分离的人外周血ＣＤ４＋细胞在佛波酯（ＰＭＡ，一种ＰＫＣ激酶活化剂）活化早期细胞表型的变化情况。我们已经研究了ＰＭＡ对人胎儿胸腺细胞表型变化的影响，发现ＰＭＡ可明显下调胸腺ＤＰ亚群ＣＤ４、ＣＤ８和ＣＤ３分子表达。但能明显促进ＣＤ２５和ＨＬＡ－Ⅰ类抗原表达。本文旨在通过研究ＰＭＡ对人外周血Ｔ细胞和ＣＤ４＋亚群表型变化的影响，探讨ＰＭＡ对发育成熟的外周血Ｔ细胞是否发挥同样的调节作用。并且通过观察外周血Ｔ细胞和ＣＤ４＋亚群对ＰＭＡ的反应性，探讨Ｔ细胞在对抗原发生应答而活化时，Ｔ细胞亚群之间是否存在某种影响细胞表型变化的相互作用。我们发现ＰＭＡ同样可下调外周血Ｔ细胞ＣＤ３ＣＤ４和ＣＤ８分子的表达，并促进ＣＤ２５分子表达。ＰＭＡ对外周血Ｔ细胞的作用与胎儿胸腺细胞相比在作用时间上有明显不同。表明处于不同发育阶段的Ｔ细胞对ｐＭＡ表现不同的反应性。另外，外周血总Ｔ细胞和ＣＤ４＋亚群细胞也对ＰＭＡ表现不同反应性，提示Ｔ细胞在ＰＭＡ作用下发生表型改变时，细胞亚群之间可能存在某种影响细胞表型变化的相互作用?
With double fluorescent-staining and
flowcytometry. we studied the phenotype variation of T cells purifiedfrom PBL with rosette
method and CD4 positive subset purified from rosette T cells with corn plement mediated
cy-totoxicity to delete CD8+ T cells. The purified T cells were cultured with PMA for different
times(0.5,3 ,6 and 12hours);We found that PMA could induce down-modulation of both CD4 and
CD8 expression on mature T cells inPBL.Compared with that of human fetal thymocytes
stimulated with PM A. however,there was obvious difference. The results suggest that T cells on
different differentiation stages may have different responses to PMA. On theother hand,we
found that the phenotypic variation between T cells and CD 4 positive subset cells in PBL had
differ-ent responses to PMA. That means the variation of T cell phenotype may be affected by
interaction of T cell sub-sets as well as be affected by PM A.It may be a very important
modulation existing in T cells activation.We also found that CPZ (Chlorpromazine )which is a
Ca++inhibitor,H7[1-(5-isoquinoline sulfonyl)-2-methylpiperrazine dihydrochloride] which is a
PKC inhibitor ,and EGT A could obviously inhibit downmodulation ofthe expression of CD3,CD4,
CD8 and CD25 by PMA.These results indicate that the flux of Ca++in T cells mayplay an
important action on differentiation and activation of T cell duing early activation。
出处
《中华微生物学和免疫学杂志》
CAS
CSCD
北大核心
1994年第3期176-179,共4页
Chinese Journal of Microbiology and Immunology
关键词
T细胞
表型分化
PMA
Tlympbocyte
Phenotypic Differentiation
PMA
