摘要
为阐明新的肿瘤标志——异常凝血酶原（ｄｅｓ－γ－Ｃａｒｂｏｘｙｐｒｏｔｈｒｏｍｂｉｎ，ＤＣＰ）在原发性肝癌（ＨＣＣ）中升高机制，本文检测了正常肝组织、肝癌组织和癌旁肝硬化组织中ＤＣＰ及总凝血酶原水平，同时测定了４株肝癌细胞系培养上清中ＤＣＰ含量。血浆ＤＣＰ升高与肝癌组织中ＤＣＰ水平相一致，癌组织中凝血酶原总量也明显增高，无论血浆中ＤＣＰ是否升高，癌旁肝硬化组织内ＤＣＰ水平均较正常组织高；４株肝癌细胞均产生ＤＣＰ，且ＤＣＰ产量随培养时间延长而增加。提示ＤＣＰ是肝癌细胞特异产物，肝癌组织中ＤＣＰ升高及凝血酶原总量的过度生成可能对肝癌血浆ＤＣＰ产生起重要作用，肝硬化组织中ＤＣＰ升高可能是一种癌前病变的标志。
We
detected the levels of DCP and immunoreactive prothrombin in various human livertissues,and
also measured the levels of DCP in the media of four human hepatoma cell linesto elucidate
the mechanism for the increase of DCP in HCC.In the patients with elevatedplasma DCP Ievels,
both DCP and immunoreactive prothrombin significantly increased inhepatoma tissues as
compared with those of cirrhosi.DCP was higher in cirrhosis ascompared with that of normal
liver tissues, irrespective of plasma DCP being positive ornegative.All four hepatoma cell lines
produced DCP in the media,that were increased in atime- dependent fashio.The results showed
that DCP was a specific product of hepatomacells,and increased DCP Ievels and overprodution
of immunoreactive prothrombin inhepatoma tissues may play an important role in the
synthesis of DCP,and cirrhotic tissueswith increased DCP contents could be precancerous.
出处
《中华消化杂志》
CAS
CSCD
北大核心
1994年第5期259-261,共3页
Chinese Journal of Digestion
关键词
异常凝血酶原
肿瘤标志
细胞系
肝肿瘤
Hepatocellular carcinoma(H CC)
Des-y-carboxy prothrombin(DCP)
Tumormarker
Tumor cell line.
